04-217 | Anti-phospho-c-Myc (Thr58/Ser62) Antibody, rabbit monoclonal

04-217
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIF, IP, WBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-217
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-c-Myc (Thr58/Ser62) Antibody, rabbit monoclonal
      Alternate Names
      • Transcription factor p64
      • avian myelocytomatosis viral oncogene homolog
      • myc proto-oncogene protein
      • v-myc avian myelocytomatosis viral oncogene homolog
      • v-myc myelocytomatosis viral oncogene homolog (avian)
      Background InformationMyc is a member of the helix-loop-helix-leucine zipper (bHLH-LZ) family of transcription factors, which dimerizes with Max to activate transcription of some genes and repress others. As Max is essential for Myc activity, the availability of Max is a regulatory mechanism for Myc activity, and members of the Mad family of proteins compete with Myc for Max.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Calyculin/Okadaic acid-treted A431 lysate
      Presentation100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationAnti-phospho-c-Myc (Thr58/Ser62) Antibody is an antibody against phospho-c-Myc (Thr58/Ser62) for use in IF, IP & WB.
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesSuggested dilutions:
      Western Blot: 1:10,000
      Immunocytochemistry: 1:50-1:100
      Immunoprecipitation: 1:100
      Biological Information
      ImmunogenSynthetic peptide corresponding to sequence surrounding and including phosphorylated Thr58 and Ser62 of human c-Myc.
      Epitopephosphorylated Thr58/Ser62
      HostRabbit
      Specificityc-Myc when phosphorylated on residues Thr58/Ser62.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
      Gene Symbol
      • c-Myc
      • C-Myc
      • MYC
      • C-MYC
      Modifications
      • Phosphorylation
      Non-Reactive Species
      • Rat
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
      SIZE: 439 amino acids; 48804 Da
      SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Phosphorylated by PRKDC.
      DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular Weight57 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-c-Myc (Thr58/Ser62), clone E203 - 2379283 2379283
      Anti-phospho-c-Myc (Thr58/Ser62), clone E203 - 2387477 2387477
      Anti-phospho-c-Myc (Thr58/Ser62), clone E203 - 2390351 2390351
      Anti-phospho-c-Myc (Thr58/Ser62), clone E203 - 2433275 2433275
      Anti-phospho-c-Myc (Thr58/Ser62), clone E203 - 2439825 2439825
      Anti-phospho-c-Myc (Thr58/Ser62), clone E203 - 1928746 1928746
      Anti-phospho-c-Myc (Thr58/Ser62), clone E203 - 1987453 1987453
      Anti-phospho-c-Myc (Thr58/Ser62), clone E203 - 2030379 2030379
      Anti-phospho-c-Myc (Thr58/Ser62), clone E203 - 2060950 2060950
      Anti-phospho-c-Myc (Thr58/Ser62), clone E203 - 2107215 2107215

      References

      Reference overviewPub Med ID
      c-Myc expression and MEK1-induced Erk2 nuclear localization are required for TGF-beta induced epithelial-mesenchymal transition and invasion in prostate cancer.
      Amatangelo, MD; Goodyear, S; Varma, D; Stearns, ME
      Carcinogenesis  33  1965-75  2012

      Show Abstract
      22791812 22791812

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