04-342 | Anti-phospho-eIF-2α (Ser51) Antibody, rabbit monoclonal

04-342
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, IH(P), WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-342
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-eIF-2α (Ser51) Antibody, rabbit monoclonal
      Alternate Names
      • EIF-2A
      References
      Product Information
      FormatPurified
      Presentation100 μL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-phospho-eIF-2α (Ser51) Antibody, rabbit is validated for use in IC, IH(P), WB for the detection of phospho-eIF-2α (Ser51).
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesSuggested Dilutions:
      Immunoblot Analysis: 1:1,000
      Immunocytochemistry: 1:100 - 1:250
      Immunohistochemistry: 1:50-1:100
      Biological Information
      ImmunogenKLH-conjugated synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2α
      HostRabbit
      SpecificityRecognizes the eIF-2α when phosphorylated on Ser51.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryEIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).[supplied by OMIM]
      Gene Symbol
      • EIF2A
      • eIF-2A
      • MSTP089
      • MSTP004
      • MST089
      • EIF-2A
      • ORFNames=CDA02
      • CDA02
      Modifications
      • Phosphorylation
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9BY44 # Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
      SIZE: 585 amino acids; 64976 Da
      TISSUE SPECIFICITY: Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
      SIMILARITY: SwissProt: Q9BY44 ## Belongs to the WD repeat EIF2A family. & Contains 3 WD repeats.
      Molecular Weight36 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment.
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-eIF-2 (Ser51) (Rabbit monoclonal IgG) - 2037226 2037226
      Anti-phospho-eIF-2 (Ser51) (Rabbit monoclonal IgG) - 2276296 2276296
      Anti-phospho-eIF-2 (Ser51) - 2051315 2051315
      Anti-phospho-eIF-2 (Ser51) - DAM1703542 DAM1703542
      Anti-phospho-eIF-2 (Ser51) - NG1866959 NG1866959
      Anti-phospho-eIF-2 (Ser51) - NG1873286 NG1873286
      Anti-phospho-eIF-2#945; (Ser51) (Rabbit monoclonal IgG) - NG1820197 NG1820197
      Anti-phospho-eIF-2alpha; (Ser51), rabbit monoclonal - 0701050227 0701050227
      Anti-phospho-eIF-2α (Ser51) - 2387731 2387731
      Anti-phospho-eIF-2α (Ser51) - 2510148 2510148

      References

      Reference overviewPub Med ID
      Tyrosine phosphorylation acts as a molecular switch to full-scale activation of the eIF2alpha RNA-dependent protein kinase
      Su, Qiaozhu, et al
      Proc Natl Acad Sci USA, 103:63-8 (2006)  2006

      16373505 16373505
      Double-stranded RNA-dependent protein kinase phosphorylation of the alpha-subunit of eukaryotic translation initiation factor 2 mediates apoptosis
      Scheuner, Donalyn, et al
      J Biol Chem, 281:21458-68 (2006)  2006

      16717090 16717090
      Proteasome inhibitor PS-341 induces apoptosis in cisplatin-resistant squamous cell carcinoma cells by induction of Noxa
      Fribley, Andrew M, et al
      J Biol Chem, 281:31440-7 (2006)  2006

      16928686 16928686
      Persistent eIF2alpha(P) is colocalized with cytoplasmic cytochrome c in vulnerable hippocampal neurons after 4 hours of reperfusion following 10-minute complete brain ischemia.
      Page, Andrea B, et al.
      Acta Neuropathol., 106: 8-16 (2003)  2003

      Show Abstract
      12687390 12687390

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies