06-229 | Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody

06-229
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RICC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-229
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-erbB-2/HER-2 (Tyr1248) Antibody
      Alternate Names
      • CD340 antigen
      • MLN 19
      • NEU proto-oncogene
      • Tyrosine kinase-type cell surface receptor HER2
      • c-erb B2/neu protein
      • neuroblastoma/glioblastoma derived oncogene homolog
      • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
      • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2,neuro/glioblastoma derived oncogene homolog (avian)
      Background InformationThe ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.
      References
      Product Information
      FormatAffinity Purified
      Control
      • EGF treated A431 cells
      PresentationDouble immunoaffinity purified rabbit polyclonal IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4
      Applications
      ApplicationThis Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody is validated for use in IC, WB for the detection of phospho-erbB-2/HER-2 (Tyr1248).
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      IHC was performed by an independent laboratory.

      Immunocytochemistry:
      2 µg/mL of a previous lot showed positive immunostaining for phospho-erbB2/HER-2 in EGF treated A431 cells.
      Biological Information
      ImmunogenSynthetic phosphopeptide, residues 1243-1255, corresponding to a major auto-phosphorylation (Tyr1248) site of human erbB-2/HER-2 (C-AENPEpYLGLDVPV).
      EpitopeTyr1248
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes and is specific for the activated form of erbB-2/HER-2 oncoprotein, MW ~138 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman. Predicted to cross react with rat based on conservation of immunogenic sequence.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
      Gene Symbol
      • ERBB2
      • HER2
      • C-erbB-2
      • NEU
      • HER-2/neu
      • NGL
      • herstatin
      • erbB-2
      • p185erbB2
      • HER-2
      • TKR1
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION:SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
      SIZE: 1255 amino acids; 137910 Da
      SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
      SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      Molecular Weight185 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on A431+/-EGF lysates.

      Western Blotting Analysis:
      1:1000 dilution of this antibody detected phospho-erbB2/Her2 on 10 µg of A431+EGF lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-erbB-2/HER-2 (Tyr1248) - 2366552 2366552
      Anti-phospho-erbB-2/HER-2 (Tyr1248) - 2398881 2398881
      Anti-phospho-erbB-2/HER-2 (Tyr1248) - 2431952 2431952
      Anti-phospho-erbB-2/HER-2 (Tyr1248) - 0701049852 0701049852
      Anti-phospho-erbB-2/HER-2 (Tyr1248) - 13320 13320
      Anti-phospho-erbB-2/HER-2 (Tyr1248) - 15916 15916
      Anti-phospho-erbB-2/HER-2 (Tyr1248) - 19941 19941
      Anti-phospho-erbB-2/HER-2 (Tyr1248) - 2017307 2017307
      Anti-phospho-erbB-2/HER-2 (Tyr1248) - 2056020 2056020
      Anti-phospho-erbB-2/HER-2 (Tyr1248) - 20871 20871

      References

      Reference overviewApplicationSpeciesPub Med ID
      S100A14, a member of the EF-hand calcium-binding proteins, is overexpressed in breast cancer and acts as a modulator of HER2 signaling.
      Xu, C; Chen, H; Wang, X; Gao, J; Che, Y; Li, Y; Ding, F; Luo, A; Zhang, S; Liu, Z
      The Journal of biological chemistry  289  827-37  2014

      Show Abstract
      Western Blotting24285542 24285542
      Hypoxia changes the expression of the epidermal growth factor (EGF) system in human hearts and cultured cardiomyocytes.
      Munk, M; Memon, AA; Goetze, JP; Nielsen, LB; Nexo, E; Sorensen, BS
      PloS one  7  e40243  2012

      Show Abstract
      22792252 22792252
      Bay846, a new irreversible small molecule inhibitor of EGFR and Her2, is highly effective against malignant brain tumor models.
      Longo, Sharon L, et al.
      Invest New Drugs, 30: 2161-72 (2012)  2012

      Show Abstract
      22203214 22203214
      Neuregulin-1β regulation of embryonic endothelial progenitor cell survival.
      Safa, RN; Peng, XY; Pentassuglia, L; Lim, CC; Lamparter, M; Silverstein, C; Walker, J; Chen, B; Geisberg, C; Hatzopoulos, AK; Sawyer, DB
      American journal of physiology. Heart and circulatory physiology  300  H1311-9  2011

      Show Abstract
      21239627 21239627
      Different changes in protein and phosphoprotein levels result from serum starvation of high-grade glioma and adenocarcinoma cell lines.
      Levin, VA; Panchabhai, SC; Shen, L; Kornblau, SM; Qiu, Y; Baggerly, KA
      Journal of proteome research  9  179-91  2010

      Show Abstract
      Western BlottingHuman19894763 19894763
      A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers.
      Hennessy, BT; Lu, Y; Gonzalez-Angulo, AM; Carey, MS; Myhre, S; Ju, Z; Davies, MA; Liu, W; Coombes, K; Meric-Bernstam, F; Bedrosian, I; McGahren, M; Agarwal, R; Zhang, F; Overgaard, J; Alsner, J; Neve, RM; Kuo, WL; Gray, JW; Borresen-Dale, AL; Mills, GB
      Clinical proteomics  6  129-51  2010

      Show Abstract Full Text Article
      21691416 21691416
      Activation of epidermal growth factor receptor ErbB1 attenuates inhibitory synaptic development in mouse dentate gyrus.
      Yuichi Abe,Hiroyuki Nawa,Hisaaki Namba
      Neuroscience research  63  2009

      Show Abstract
      19071166 19071166
      Impaired PTPN13 phosphatase activity in spontaneous or HPV-induced squamous cell carcinomas potentiates oncogene signaling through the MAP kinase pathway.
      Hoover, AC; Strand, GL; Nowicki, PN; Anderson, ME; Vermeer, PD; Klingelhutz, AJ; Bossler, AD; Pottala, JV; Hendriks, WJ; Lee, JH
      Oncogene  28  3960-70  2009

      Show Abstract
      19734941 19734941
      Heregulin beta1 drives gefitinib-resistant growth and invasion in tamoxifen-resistant MCF-7 breast cancer cells.
      Hutcheson, IR; Knowlden, JM; Hiscox, SE; Barrow, D; Gee, JM; Robertson, JF; Ellis, IO; Nicholson, RI
      Breast cancer research : BCR  9  R50  2007

      Show Abstract Full Text Article
      17686159 17686159
      Notch signaling is essential for ventricular chamber development.
      Grego-Bessa, J; Luna-Zurita, L; del Monte, G; Bolós, V; Melgar, P; Arandilla, A; Garratt, AN; Zang, H; Mukouyama, YS; Chen, H; Shou, W; Ballestar, E; Esteller, M; Rojas, A; Pérez-Pomares, JM; de la Pompa, JL
      Developmental cell  12  415-29  2007

      Show Abstract
      Western Blotting17336907 17336907

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies