04-293 | Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8

04-293
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, MELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-293
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-erbB2 (Thr686) Antibody, clone 7F8
      Alternate Names
      • NEU
      • NGL
      • HER2
      • TKR1
      • HER-2
      • c-erb B2
      References
      Product Information
      FormatPurified
      Control
      • Includes EGF treated A431 cell lysates as positive control
      PresentationIn 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol.
      Applications
      ApplicationAnti-phospho-erbB2 (Thr686) Antibody, clone 7F8 is an antibody against phospho-erbB2 (Thr686) for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 686 in human erbB2
      Clone7F8
      HostMouse
      SpecificityRecognizes phosphorylated erbB2 on Threonine 686
      IsotypeIgG1
      Species Reactivity
      • Canine
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
      Gene Symbol
      • ERBB2
      • HER2
      • HER-2
      • NGL
      • herstatin
      • erbB-2
      • C-erbB-2
      • NEU
      • TKR1
      • p185erbB2
      • HER-2/neu
      Modifications
      • Phosphorylation
      Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
      SIZE: 1255 amino acids; 137910 Da
      SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
      SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      Molecular Weight180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-erbB2 (Thr686), clone 7F8 (Mouse monoclonal IgG1) 2988851
      Anti-phospho-erbB2 (Thr686), clone 7F8 (Mouse monoclonal IgG1) 2910369
      Anti-phospho-erbB2 (Thr686), clone 7F8 (Mouse monoclonal IgG1) - 2061581 2061581
      Anti-phospho-erbB2 (Thr686), clone 7F8 - DAM1485118 DAM1485118
      Anti-phospho-erbB2 (Thr686), clone 7F8 - DAM1794016 DAM1794016
      Anti-phospho-erbB2 (Thr686), clone 7F8 - DAM1805278 DAM1805278
      Anti-phospho-erbB2 (Thr686), clone 7F8 -2638054 2638054
      Anti-phospho-erbB2 (Thr686), clone 7F8 -2707856 2707856

      References

      Reference overviewPub Med ID
      The duration of phorbol-inducible ErbB2 tyrosine dephosphorylation parallels that of receptor endocytosis rather than threonine-686 phosphorylation: implications for the physiological role of protein kinase C in growth factor receptor signalling
      Ouyang, X, et al
      Carcinogenesis, 19:2013-9 (1998)  1998

      9855018 9855018
      Human cancer cells exhibit protein kinase C-dependent c-erbB-2 transmodulation that correlates with phosphatase sensitivity and kinase activity
      Ouyang, X, et al
      J Biol Chem, 271:21786-92 (1996)  1996

      8702975 8702975

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies