09-272 | Anti-phospho-p38α (Thr180/Tyr182) Antibody

09-272
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-272
      ReplacesAB3828
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-p38α (Thr180/Tyr182) Antibody
      Alternate Names
      • SAPK2a
      • MAPK14
      • CSBP1
      • CSBP2
      • CSPB1
      • PRKM14
      • PRKM15
      • p38ALPHA
      • mitogen-activated protein kinase 14
      References
      Product Information
      FormatAffinity Purified
      Control
      • Anisomycin treated HeLa cells
      PresentationPurified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.
      Applications
      ApplicationThis Anti-phospho-p38α (Thr180/Tyr182) Antibody is validated for use in IP, WB for the detection of phospho-p38α (Thr180/Tyr182).
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoblot Analysis: A 1:1,000 dilution of the lot detected p38α dually phosphorylated on residues Thr180 and Tyr182 in lysate from HeLa cells stimulated with anisomycin.

      Immunfluorescence: A 1:100 dilution of this antibody detected p38α phosphorylated on both residues, Thr180 and Tyr182, in fixed HeLa cells stimulated with anisomycin.

      Immunoprecipitation (IP): 2-5 μL (5-10 μL/mL) of this antibody immunoprecipitated both active p38α from anisomycin treated HeLa whole cell extract and human recombinant GST-p38α, separately, in RIPA buffer.

      Immunoprecipitation Kinase Reaction (IPK): 2-5 μL of this lot immunoprecipitated phosphorylated p38α from both anisomycin treated HeLa cells as well as recombinant p38α, separately, and then placed into an in vitro Kinase reaction with recombinant GST-ATF-2 where it phosphorylated ATF-2 on Thr69/71.
      Biological Information
      ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-p38α (Thr180/Tyr182).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes p38α dual phosphorylation of Thr180 and Tyr182.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
      Gene Symbol
      • MAPK14
      • PRKM14
      • RK
      • SAPK2A
      • CSBP1
      • p38
      • CSPB1
      • CSBP
      • PRKM15
      • Mxi2
      • p38ALPHA
      • MXI2
      • EXIP
      • CSBP2
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16539 # Responds to activation by environmental stress, pro- inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
      COFACTOR: Magnesium.
      SIZE: 360 amino acids; 41293 Da
      SUBUNIT: Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation. Interacts with SPAG9 (By similarity). Interacts with NP60.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).
      TISSUE SPECIFICITY: Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.DOMAIN:SwissProt: Q16539 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. & Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-p38 (Thr180/Tyr182) - DAM1564582 DAM1564582
      Anti-phospho-p38a (Thr180/Tyr182) (Affinity purified Rabbit polyclonal IgG) - 1966948 1966948
      Anti-phospho-p38a (Thr180/Tyr182) (Affinity purified Rabbit polyclonal IgG) - 2243500 2243500
      Anti-phospho-p38a (Thr180/Tyr182) - 2054866 2054866
      Anti-phospho-p38a (Thr180/Tyr182) - DAM1503386 DAM1503386
      Anti-phospho-p38a (Thr180/Tyr182) - DAM1601848 DAM1601848
      Anti-phospho-p38a (Thr180/Tyr182) - DAM1655273 DAM1655273
      Anti-phospho-p38a (Thr180/Tyr182) - JBC1821163 JBC1821163
      Anti-phospho-p38α (Thr180/Tyr182) (Affinity purified Rabbit polyclonal IgG) 2982404
      Anti-phospho-p38α (Thr180/Tyr182) (Affinity purified Rabbit polyclonal IgG) -2500553 2500553

      References

      Reference overviewPub Med ID
      A high-quality secretome of A549 cells aided the discovery of C4b-binding protein as a novel serum biomarker for non-small cell lung cancer.
      Xiaoyang Luo,Yansheng Liu,Rui Wang,Haichuan Hu,Rong Zeng,Haiquan Chen
      Journal of proteomics  74  2011

      Show Abstract
      21262398 21262398
      Loss of cystic fibrosis transmembrane conductance regulator function enhances activation of p38 and ERK MAPKs, increasing interleukin-6 synthesis in airway epithelial cells exposed to Pseudomonas aeruginosa.
      Bérubé, J; Roussel, L; Nattagh, L; Rousseau, S
      The Journal of biological chemistry  285  22299-307  2010

      Show Abstract
      20460375 20460375
      Distinct intracellular signaling pathways control the synthesis of IL-8 and RANTES in TLR1/TLR2, TLR3 or NOD1 activated human airway epithelial cells.
      Julie Bérubé,Céline Bourdon,Yu Yao,Simon Rousseau
      Cellular signalling  21  2009

      Show Abstract
      19121387 19121387
      p38 MAPK regulates phosphorylation of Bad via PP2A-dependent suppression of the MEK1/2-ERK1/2 survival pathway in TNF-alpha induced endothelial apoptosis
      Grethe, Simone and Porn-Ares, M Isabella
      Cell Signal, 18:531-40 (2006)  2006

      15972258 15972258
      Role of Rho/ROCK and p38 MAP kinase pathways in transforming growth factor-beta-mediated Smad-dependent growth inhibition of human breast carcinoma cells in vivo
      Kamaraju, Anil K and Roberts, Anita B
      J Biol Chem, 280:1024-36 (2005)  2005

      15520018 15520018
      Targeted inhibition of p38alpha MAPK suppresses tumor-associated endothelial cell migration in response to hypericin-based photodynamic therapy
      Hendrickx, Nico, et al
      Biochem Biophys Res Commun, 337:928-35 (2005)  2005

      16214108 16214108
      Induction of terminal differentiation by constitutive activation of p38 MAP kinase in human rhabdomyosarcoma cells
      Puri, P L, et al
      Genes Dev, 14:574-84 (2000)  2000

      10716945 10716945
      Identification of a dual specificity kinase that activates the Jun kinases and p38-Mpk2
      Lin, A, et al
      Science, 268:286-90 (1995)  1995

      7716521 7716521

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies