04-745 | Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, DB, Mplex, ChIP-seq, IH(P)RbCulture SupernatantMonoclonal Antibody
      Catalogue Number04-745
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal
      Alternate Names
      • H3K4me3
      • Histone H3 (tri methyl K4)
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      FormatCulture Supernatant
      • HeLa acid extract
      PresentationCultured supernantant in 0.05% sodium azide.
      ApplicationAnti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315 is a rabbit monoclonal antibody for detection of trimethyl-Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in WB, ChIP, DB, Mplex, ChIP-seq.
      Key Applications
      • Western Blotting
      • Dot Blot
      • Multiplexing
      • ChIP-seq
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in human colon and human breast cancer tissue sections
      Biological Information
      ImmunogenPeptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
      SpecificityHistone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See COA for specificity data.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3.4
      • H3T
      • H3/g
      • H3/t
      • Methylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on in acid extracted proteins from HeLa cells, but not recombinant unmethylated Histone H3 (Catalog #14-494)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-trimethyl-Histone H3 (Lys4) 2462884
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2135147 2135147
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2326991 2326991
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2392291 2392291
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2452485 2452485
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - NG1823938 NG1823938
      Anti-trimethyl-Histone H3 (Lys4), -2591879 2591879
      Anti-trimethyl-Histone H3 (Lys4), -2647455 2647455
      Anti-trimethyl-Histone H3 (Lys4), -2664238 2664238
      Anti-trimethyl-Histone H3 (Lys4), -2700777 2700777


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      Epigenetics & chromatin  8  39  2015

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      Tyler, CR; Weber, JA; Labrecque, M; Hessinger, JM; Edwards, JS; Allan, AM
      Data in brief  5  248-54  2015

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      Hierarchical clustering of breast cancer methylomes revealed differentially methylated and expressed breast cancer genes.
      Lin, IH; Chen, DT; Chang, YF; Lee, YL; Su, CH; Cheng, C; Tsai, YC; Ng, SC; Chen, HT; Lee, MC; Chen, HW; Suen, SH; Chen, YC; Liu, TT; Chang, CH; Hsu, MT
      PloS one  10  e0118453  2015

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      T-cell receptor α enhancer is inactivated in αβ T lymphocytes.
      del Blanco, B; Angulo, Ú; Krangel, MS; Hernández-Munain, C
      Proceedings of the National Academy of Sciences of the United States of America  112  E1744-53  2015

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      Immunoprecipitation25831496 25831496

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