07-463 Anti-trimethyl-Histone H4 (Lys20) Antibody

07-463
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, DB, ChIPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-463
      DescriptionAnti-trimethyl-Histone H4 (Lys20) Antibody
      Alternate Names
      • Histone H4 (trimethyl Lys20)
      • H4K20me3
      Background InformationHistones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa acid extract and various recombinant Histone proteins.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-trimethyl-Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of trimethyl-Histone H4 (Lys20) also known as H4K20me3, Histone H4 (tri methyl K20) & has been validated in IP, WB, ChIP, DB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Dot Blot
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blot Analysis: 2 µg/mL from a representative lot detected trimethyl-Histone H4 (Lys20) in 10 µg of BALB3T3/A31 acid extract and RAT2 acid extract.

      Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see tables) were probed with Anti-trimethyl-Histone H4 (Lys20) (4 µg/mL).

      Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H4 using chromatin from HeLa cells.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated trimethyl-Histone H4 (Lys20) from HeLa cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4 trimethylated at Lys20.
      EpitopeTrimethylated Lys20
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the N-terminus of Histone H4 trimethylated at Lys20.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/C
      • H4/D
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~13 kDa observed. An uncharacterized band may appear at ~110 kDa in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.

      Western Blot Analysis: 2 µg/mL of this antibody detected trimethyl-Histone H4 (Lys20) in 10 µg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-trimethyl-Histone H4 (Lys20) (rabbit antiserum) - DAM1462568DAM1462568
      Anti-trimethyl-Histone H4 (Lys20) - 21677882167788
      Anti-trimethyl-Histone H4 (Lys20) - 23835332383533
      Anti-trimethyl-Histone H4 (Lys20) - 19773771977377
      Anti-trimethyl-Histone H4 (Lys20) - 20545192054519
      Anti-trimethyl-Histone H4 (Lys20) - 21931622193162
      Anti-trimethyl-Histone H4 (Lys20) - 22972322297232
      Anti-trimethyl-Histone H4 (Lys20) - 2447524475
      Anti-trimethyl-Histone H4 (Lys20) - 24940492494049
      Anti-trimethyl-Histone H4 (Lys20) - 3139231392

      References

      Reference overviewApplicationSpeciesPub Med ID
      Intracellular α-ketoglutarate maintains the pluripotency of embryonic stem cells.
      Carey, BW; Finley, LW; Cross, JR; Allis, CD; Thompson, CB
      Nature  518  413-6  2015

      Show Abstract
      25487152 25487152
      Deep sequencing and de novo assembly of the mouse oocyte transcriptome define the contribution of transcription to the DNA methylation landscape.
      Veselovska, L; Smallwood, SA; Saadeh, H; Stewart, KR; Krueger, F; Maupetit-Méhouas, S; Arnaud, P; Tomizawa, S; Andrews, S; Kelsey, G
      Genome biology  16  209  2015

      Show Abstract
      26408185 26408185
      IL-22(+)CD4(+) T cells promote colorectal cancer stemness via STAT3 transcription factor activation and induction of the methyltransferase DOT1L.
      Kryczek, I; Lin, Y; Nagarsheth, N; Peng, D; Zhao, L; Zhao, E; Vatan, L; Szeliga, W; Dou, Y; Owens, S; Zgodzinski, W; Majewski, M; Wallner, G; Fang, J; Huang, E; Zou, W
      Immunity  40  772-84  2014

      Show Abstract
      24816405 24816405
      Hypermethylation of the alternative AWT1 promoter in hematological malignancies is a highly specific marker for acute myeloid leukemias despite high expression levels.
      Guillaumet-Adkins, A; Richter, J; Odero, MD; Sandoval, J; Agirre, X; Catala, A; Esteller, M; Prósper, F; Calasanz, MJ; Buño, I; Kwon, M; Court, F; Siebert, R; Monk, D
      Journal of hematology & oncology  7  4  2014

      Show Abstract
      Human24405639 24405639
      Epstein-Barr virus-mediated transformation of B cells induces global chromatin changes independent to the acquisition of proliferation.
      Hernando, H; Islam, AB; Rodríguez-Ubreva, J; Forné, I; Ciudad, L; Imhof, A; Shannon-Lowe, C; Ballestar, E
      Nucleic acids research  42  249-63  2014

      Show Abstract
      Western Blotting24097438 24097438
      Histone H3.3 and its proteolytically processed form drive a cellular senescence programme.
      Duarte, LF; Young, AR; Wang, Z; Wu, HA; Panda, T; Kou, Y; Kapoor, A; Hasson, D; Mills, NR; Ma'ayan, A; Narita, M; Bernstein, E
      Nature communications  5  5210  2014

      Show Abstract
      25394905 25394905
      Cerebellar oxidative DNA damage and altered DNA methylation in the BTBR T+tf/J mouse model of autism and similarities with human post mortem cerebellum.
      Shpyleva, S; Ivanovsky, S; de Conti, A; Melnyk, S; Tryndyak, V; Beland, FA; James, SJ; Pogribny, IP
      PloS one  9  e113712  2014

      Show Abstract
      Western Blotting25423485 25423485
      Suv4-20h2 mediates chromatin compaction and is important for cohesin recruitment to heterochromatin.
      Hahn, M; Dambacher, S; Dulev, S; Kuznetsova, AY; Eck, S; Wörz, S; Sadic, D; Schulte, M; Mallm, JP; Maiser, A; Debs, P; von Melchner, H; Leonhardt, H; Schermelleh, L; Rohr, K; Rippe, K; Storchova, Z; Schotta, G
      Genes & development  27  859-72  2013

      Show Abstract
      23599346 23599346
      Distinctive patterns of epigenetic marks are associated with promoter regions of mouse LINE-1 and LTR retrotransposons.
      Rangasamy, D
      Mobile DNA  4  27  2013

      Show Abstract
      24289137 24289137
      Naive pluripotency is associated with global DNA hypomethylation.
      Leitch, HG; McEwen, KR; Turp, A; Encheva, V; Carroll, T; Grabole, N; Mansfield, W; Nashun, B; Knezovich, JG; Smith, A; Surani, MA; Hajkova, P
      Nature structural & molecular biology  20  311-6  2013

      Show Abstract
      Western Blotting23416945 23416945

      Brochure

      Title
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
      Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

      Posters

      Title
      Histone Modifications

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