07-463-S | Anti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size

07-463-S
10 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, DB, ChIPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-463-S
      DescriptionAnti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size
      Alternate Names
      • H4K20me3
      • Histone H4 (trimethyl K20)
      • H4 histone family, member A
      • Histone 1, H4a
      • histone cluster 1, H4a
      Background InformationHistones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa acid extract and various recombinant Histone proteins.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size/ Pack is a rabbit polyclonal antibody for detection of trimethyl-Histone H4 (Lys20) also known as H4K20me3, Histone H4 (tri methyl K20) & has been validated in IP, WB, ChIP, DB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Dot Blot
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blot Analysis: 2 µg/mL from a representative lot detected trimethyl-Histone H4 (Lys20) in 10 µg of BALB3T3/A31 acid extract and RAT2 acid extract.

      Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see tables) were probed with Anti-trimethyl-Histone H4 (Lys20) (4 µg/mL).

      Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H4 using chromatin from HeLa cells.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated trimethyl-Histone H4 (Lys20) from HeLa cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4 trimethylated at Lys20.
      EpitopeTrimethylated Lys20
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the N-terminus of Histone H4 trimethylated at Lys20.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/C
      • H4/D
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~13 kDa observed. An uncharacterized band may appear at ~110 kDa in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.

      Western Blot Analysis: 2 µg/mL of this antibody detected trimethyl-Histone H4 (Lys20) in 10 µg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-trimethyl Histone H4 (Lys20), -2702117 2702117
      Anti-trimethyl Histone H4 (Lys20), Trial Size - 2420549 2420549
      Anti-trimethyl Histone H4 (Lys20), Trial Size - Q2243191 Q2243191
      Anti-trimethyl Histone H4 (Lys20), Trial Size -2596509 2596509
      Anti-trimethyl Histone H4 (Lys20), Trial Size -2617691 2617691
      Anti-trimethyl Histone H4 (Lys20), Trial Size -2623694 2623694
      Anti-trimethyl Histone H4 (Lys20), Trial Size Polyclonal Antibody 2972040

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies