APT110 | Apo-Direct TUNEL Assay Kit

APT110
50 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsDetection Methods
      FCFluorescent
      Description
      Catalogue NumberAPT110
      Brand Family Chemicon®
      Trade Name
      • ApoDIRECT
      • Chemicon
      DescriptionApo-Direct TUNEL Assay Kit
      OverviewThe CHEMICON APO-DIRECT™ Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.
      Materials Required but Not Delivered1. Flow Cytometer

      2. Distilled Water

      3. 1% (w/v) paraformaldehyde (methanol free) in PBS

      4. 70% (v/v) ethanol

      5. 37oC Water Bath

      6. Ice Bucket

      7. 12 x 75 mm flow cytometry test tubes

      8. Micro-pipettor
      References
      Product Information
      Components
      • The following components are included with the APO-DIRECT™ Kit. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 50 cell suspensions including control cells of approximately 1 x 106 cells per suspension for flow cytometry analysis. The control cells have been fixed as described (under the heading cell fixation procedure) and are in 70% (v/v) ethanol.
      • Positive Control Cells,Brown cap
      • Negative Control Cells, Natural cap
      • Wash Buffer, Blue cap
      • Reaction Buffer, Green cap
      • TdT Enzyme, Yellow cap
      • F-dUTP, Orange cap
      • Rinsing Buffer, Red cap
      • PI/RNase Staining Buffer, Amber bottle
      Detection methodFluorescent
      Applications
      ApplicationThe APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • Vertebrates
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsPrecautions and Warnings:

      1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.

      2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets.

      3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.
      Packaging Information
      Material Size50 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Splicing factor SRSF3 is crucial for hepatocyte differentiation and metabolic function.
      Sen, Supriya, et al.
      Nat Commun, 4: 1336 (2013)  2013

      Show Abstract
      23299886 23299886
      High glucose induces suppression of insulin signalling and apoptosis via upregulation of endogenous IL-1beta and suppressor of cytokine signalling-1 in mouse pancreatic beta cells.
      Venieratos, Panagiotis D, et al.
      Cellular signalling, (2010)  2010

      Show Abstract
      20067833 20067833
      The protease-mediated nucleus shuttles of subnanometer gold quantum dots for real-time monitoring of apoptotic cell death.
      Shu-Yi Lin,Nai-Tzu Chen,Shu-Pin Sun,Jerry C Chang,Yu-Chao Wang,Chung-Shi Yang,Leu-Wei Lo
      Journal of the American Chemical Society  132  2010

      Show Abstract
      20499915 20499915

      User Guides

      Title
      Apo-Direct TUNEL Assay Kit

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Apoptosis / Cell Death Assays