ES006-500ML BCIP/NBT Single Reagent, Blue/purple, Alkaline Phosphatase Substrate (slightly soluble in alcohol)

ES006-500ML
500 mL  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      WB, IHC
      Description
      Catalogue NumberES006-500ML
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionBCIP/NBT Single Reagent, Blue/purple, Alkaline Phosphatase Substrate (slightly soluble in alcohol)
      OverviewThe most sensitive alkaline phosphatase substrate available for blotting techniques and in situ hybridization chemistry is the 5-bromo-4-chloro-3-indolyl phosphate/p-nitro blue tetrazolium system (BCIP/NBT). Using proprietary production and stabilization procedures, CHEMICON has made available a unique BCIP/NBT reagent that is stable at room temperature and several times more sensitive than existing formulations. For localization of enzyme labeled probes following Western, Northern, Southern or dot blotting, the reagent is added directly to the membrane. Following a 10-15 minute incubation, the reaction is terminated by distilled water rinses. Longer incubations may be employed if necessary, but use of biotin/streptavidin-alkaline phosphatase signaling complexes will negate prolonged reaction times.
      References
      Product Information
      Presentation5-bromo-4-chloro-3-indolyl phosphate p-nitro blue tetrazolium chloride in a proprietary buffer and enhancer.
      Color CodeBlack
      Applications
      Application500 ml BCIP/NBT Single Reagent, Blue/purple, Alkaline Phosphatase Substrate (slightly soluble in alcohol) for Western Blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at room temperature or 2-8°C for up to one year.
      Packaging Information
      Material Size500 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      BCIP/NBT -27925222792522
      BCIP/NBT -28034992803499
      BCIP/NBT ALKALINE PHOSPHATASE SUBSTRATE3132981
      BCIP/NBT ALKALINE PHOSPHATASE SUBSTRATE3153318
      BCIP/NBT ALKALINE PHOSPHATASE SUBSTRATE2919795
      BCIP/NBT ALKALINE PHOSPHATASE SUBSTRATE3066211
      BCIP/NBT ALKALINE PHOSPHATASE SUBSTRATE3073330
      BCIP/NBT ALKALINE PHOSPHATASE SUBSTRATE2972454
      BCIP/NBT ALKALINE PHOSPHATASE SUBSTRATE2960610
      BCIP/NBT ALKALINE PHOSPHATASE SUBSTRATE2887769

      References

      Reference overviewPub Med ID
      Detection of sub-picogram quantities of specific DNA sequences on blot hybridization with biotinylated probes.
      Chan, V T, et al.
      Nucleic Acids Res., 13: 8083-91 (1985)  1985

      Show Abstract
      2933635 2933635
      Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.
      Leary, J J, et al.
      Proc. Natl. Acad. Sci. U.S.A., 80: 4045-9 (1983)  1983

      Show Abstract
      6191328 6191328