03-231 | Beclin 1 (Human) Recombinant Protein

20 µg  
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      Replacement Information
      Catalogue Number03-231
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionBeclin 1 (Human) Recombinant Protein
      Background InformationThe Beclin-1 (BECN1) gene encodes a coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson's and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia. It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport.
      Product Information
      • 1 vial of 20 µg
      • Human Beclin 1 recombinant protein in 20 mM Tris-HCl pH 8.0, containing 150 mM NaCl, protease inhibitor cocktail and 10% glycerol.
      Application NotesWestern Blot Analysis
      A 1:1000 dilution of Cat. No. MABC139, Anti-Beclin-1, clone 4E10.1 was used to detect 0.05 µg of Human Beclin-1 recombinant protein (Cat. No. 03-231). Proteins were visualized using a Goat Anti-Mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

      Purified Human Beclin 1 protein gel
      0.05 µg of Human Beclin 1 recombinant protein was loaded onto 10-20% Bis-Tris gel and stained with ponceau.
      Biological Information
      ConcentrationSee datasheet for lot-specific concentration.
      Purity≥99% (SDS-PAGE)
      Gene Symbol
      • BECN1
      • GT197
      UniProt Number
      UniProt SummaryFunction:Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
      Subunit structure: Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy By similarity. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB By similarity. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1.
      Subcellular location: Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Note: Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells
      Tissue specificity: Ubiquitous.
      Post-translational modification: Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.Polyubiquitinated by NEDD4, both with 'Lys11'- and 'Lys63'-linkages. 'Lys'-11-linked poyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes.
      Sequence similarities: Belongs to the beclin family.
      Molecular Weight~56 kDa observed.
      Uncharacterized bands may be observed.
      Calculated molecular weight of 52 kDa.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -80ºC for up to 3 months after date of receipt.
      Avoid freeze/thaw cycles.
      Packaging Information
      Material Size20 µg
      Transport Information
      Supplemental Information