For gentle, efficient, non-mechanical extraction of soluble proteins from bacterial cells, use BugBuster® Protein Extraction Reagents. This proprietary, innovative, Tris-buffer based mixture of non-ionic and zwitterionic detergents and other ingredients is capable of perforating cell walls without denaturing protein. It provides a simple, rapid, low-cost alternative to mechanical methods, such as French Press or sonication, for releasing expressed target protein for purification or other applications. Simply add the BugBuster® Reagent to your cell pellet and incubate at room temperature for 10 minutes.
Features & Benefits
No special sample preparation or instruments are needed
Standard reagent is supplied as a Tris-buffered "1X" ready-to-use liquid
Stable at room temperature
Performance can be enhanced using Benzonase® Nuclease and rLysozyme™ Solution
Fully compatible with GST•Bind™, Ni-NTA His•Bind®, S•Tag™, Strep•Tactin, and T7•Tag® Resins, or several other chromatography matrices for affinity purification
Protein Extraction, Protein Sample Preparation, Bacterial Cell Lysis