12-373 | CHKtide (CHK1/CHK2 substrate peptide)

12-373
1 mg  
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      Overview

      Replacement Information
      Description
      Catalogue Number12-373
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionCHKtide (CHK1/CHK2 substrate peptide)
      References
      Product Information
      PresentationLyophilized powder
      Applications
      ApplicationCHKtide (CHK1/CHK2 substrate peptide) primarily used in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryIn response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • CHEK1
      • CHK1
      Protein TargetCHK
      Target Sub-FamilyCAMK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O96017 # Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
      COFACTOR: Magnesium.
      SIZE: 543 amino acids; 60915 Da
      SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. & Isoform 4: Nucleus. & Isoform 7: Nucleus. & Isoform 9: Nucleus. & Isoform 12: Nucleus.
      TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
      DISEASE: SwissProt: O96017 # Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. & Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. & Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].
      SIMILARITY: SwissProt: O96017 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. & Contains 1 FHA domain. & Contains 1 protein kinase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Protein Kinase Assay as a substrate for the active CHK1 (Catalog #14-346) and CHK2 (Catalog #14-347) kinases.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsLyophilized: 2 years at 4°C; Rehydrated: 6 months at -20°C.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      CHKtide Substrate Peptide 2953824
      CHKtide Substrate Peptide 3028079
      CHKtide Substrate Peptide 3059115
      CHKtide Substrate Peptide 2902704
      CHKtide Substrate Peptide - 2343569 2343569
      CHKtide Substrate Peptide - 2535287 2535287
      CHKtide Substrate Peptide - DAM1724059 DAM1724059
      CHKtide Substrate Peptide - DAM1794313 DAM1794313
      CHKtide Substrate Peptide - DAM1823414 DAM1823414
      CHKtide Substrate Peptide - JBC1863378 JBC1863378

      References

      Reference overviewApplicationPub Med ID
      Telomere attrition and Chk2 activation in human heart failure
      Oh, H., et al
      Proc Natl Acad Sci U S A, 100:5378-83 (2003)  2003

      Kinase Assay12702777 12702777
      Cdc25 mitotic inducer targeted by chk1 DNA damage checkpoint kinase.
      Furnari, B, et al.
      Science, 277: 1495-7 (1997)  1997

      Show Abstract
      9278510 9278510
      Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25.
      Sanchez, Y, et al.
      Science, 277: 1497-501 (1997)  1997

      Show Abstract
      9278511 9278511

      Data Sheet

      Title
      CHKtide

      Related Products & Applications

      Alternative Format

      Catalogue Number Description  
      12-414 CHKtide, biotin conjugate Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Kinase & Phosphatase Substrates
      Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Kinase & Phosphatase Substrates