EZCL-31K Canine Leptin ELISA

EZCL-31K
96-well strip plate  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      LeptinCaELISAColorimetric, Fluorescent
      Description
      Catalogue NumberEZCL-31K
      DescriptionCanine Leptin ELISA
      Background InformationLeptin, a 16 kD protein and a product of the obesity (ob) gene, is synthesized and secreted predominantly by adipose tissue and is, therefore, considered an adipokine. With circulating levels directly proportional to the total amount of body fat, leptin plays an important role in whole body metabolism, regulating food intake and energy balance, as well as assisting in the control of homeostasis, reproductive function, the immune system and numerous other body functions. Binding to receptors of the hypothalamus, leptin signals food satiety and reduces appetite, although evidence suggests that, in general, obese people may have unusually high circulating levels of leptin. This may indicate a resistance to leptin similar to the insensitivity to insulin associated with type 2 diabetes. Consequently, the study of leptin is essential to understanding the relationship among appetite, energy balance and obesity.
      References
      Product Information
      Detection methodColorimetric Fluorescent
      Precision, %
      • Inter-assay: 6% - 7%
      • Intra-assay: 2% - 6%
      Applications
      ApplicationThis Canine Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • ELISA
      Application NotesThis Is a 4-hour room temperature assay using 20 μL sample size
      Biological Information
      Species Reactivity
      • Canine
      Analytes Available
      • Leptin
      Accuracy
      • 100 - 106%
      Physicochemical Information
      Sensitivity
      • 0.21 ng/mL
      Linearity of DilutionContact Technical Support.
      Standard Curve Range
      • 0.78 ng/mL - 50 ng/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: Canine Leptin 96-Well Plate Assay

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Effect of trans-fat, fructose and monosodium glutamate feeding on feline weight gain, adiposity, insulin sensitivity, adipokine and lipid profile.
      Collison KS, Zaidi MZ, Saleh SM, Inglis A, Mondreal R, Makhoul NJ, Bakheet R, Burrows J, Milgram NW, Al-Mohanna FA
      The British journal of nutrition  2011

      Show Abstract
      21429276 21429276

      Brochure

      Title
      Custom Immunoassay Menu of Services
      ELISAs, RIAs & GyroMark HT Assays

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Quality Control Range - Canine Leptin ELISA Kit

      Data Sheet

      Title
      Full Service Custom-built Assays

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits