APT105 | CaspScreen™, non-adherent cells only

APT105
25 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsDetection Methods
      MaFC, ACTFluorescent
      Description
      Catalogue NumberAPT105
      Brand Family Chemicon®
      Trade Name
      • CaspSCREEN
      • Chemicon
      DescriptionCaspScreen™, non-adherent cells only
      OverviewActivation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CaspSCREEN™ Flow Cytometric Apoptosis Detection Kit provides a simple and convenient means for detecting activation of caspases by flow cytometry in intact cells. The assay utilizes a substrate that contains two aspartate residues linked to rhodamine 110 (D2R), a reported substrate for members of capase family proteases. The rhodamine 110 conjugated substrate is non-fluorescent, however, upon cleavage of the substrate by cellular caspases or apoptosis-related proteases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm. As the D2R substrate is more cell-permeable than other fluorometric caspase substrates, apoptosis can easily be measured in intact cells by flow cytometry.
      Materials Required but Not Delivered· Microcentrifuge and 1.5 mL Microcentrifuge tubes

      · 37°C water bath or incubator

      · Flow Cytometer
      References
      Product Information
      Components
      • D(2)R - 25 μL
      • DTT (1 M): 75 μL
      • Incubation Buffer: 7.5 mL
      Detection methodFluorescent
      Applications
      ApplicationThe CaspSCREEN Flow Cytometric Apoptosis Detection Kit for Flow Cytometry provides a simple & convenient means for detecting activation of caspases by flow cytometry in intact cells.
      Key Applications
      • Flow Cytometry
      • Activity Assay
      Biological Information
      Species Reactivity
      • Mammals
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWe recommend storing reagents at -20°C upon arrival. (Store Incubation Buffer at 4°C after opening). The performance of this product is guaranteed for 6 months.
      Packaging Information
      Material Size25 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overviewPub Med ID
      Density gradient centrifugation before or after magnetic-activated cell sorting: which technique is more useful for clinical sperm selection?
      M Tavalaee,M R Deemeh,M Arbabian,M H Nasr-Esfahani
      Journal of assisted reproduction and genetics  29  2012

      Show Abstract
      22170231 22170231
      HSV and glycoprotein J inhibit caspase activation and apoptosis induced by granzyme B or Fas.
      Jerome, K R, et al.
      J. Immunol., 167: 3928-35 (2001)  2001

      Show Abstract
      11564811 11564811

      User Guides

      Title
      CaspSCREEN, non-adherent cells only

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      Categories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Caspase & Apoptosis Detection