APT161 | Caspase 1 Colorimetric Assay Kit, YVAD

APT161
100 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsDetection Methods
      MaACTChromogenic
      Description
      Catalogue NumberAPT161
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionCaspase 1 Colorimetric Assay Kit, YVAD
      OverviewActivation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's ICE/Caspase-1 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-1 activity.

      For research use only; Not for use in diagnostic procedures
      Alternate Names
      • ICE
      Materials Required but Not Delivered· Microcentrifuge and 1.5 mL Microcentrifuge tubes

      · 37°C water bath or incubator

      · Spectrophotometer

      · 96 well plate
      References
      Product Information
      Components
      • Cell Lysis Buffer - 100 mL
      • 2X Reaction Buffer - 4 x 2 mL.
      • DTT (1 M): 400 μL
      • YVAD-pNA Substrate: 500 μL
      • Dilution Buffer: 100 mL
      Detection methodChromogenic
      Applications
      ApplicationThe ICE/Caspase-1 Colorimetric Protease Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence YVAD.
      Key Applications
      • Activity Assay
      Biological Information
      Species Reactivity
      • Mammals
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms.
      Gene Symbol
      • CASP1
      • IL1BC
      • p45
      • ICE
      • IL1BCE
      • P45
      • IL-1BC
      • IL1B-convertase
      • CASP-1
      • EC 3.4.22.36 [Contains: Caspase-1 subunit p20
      • Caspase-1 subunit p10].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29466 # Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
      SIZE: 404 amino acids; 45159 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
      PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
      SIMILARITY: SwissProt: P29466 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWe recommend storing reagents at -20°C upon arrival. (Store Cell Lysis Buffer and 2X Reaction Buffer and dilution buffer at 4°C after opening). The performance of this product is guaranteed for 6 months.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications