APT163 | Caspase 2 Colorimetric Assay Kit, VDVAD

APT163
100 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsDetection Methods
      MaACTChromogenic
      Description
      Catalogue NumberAPT163
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionCaspase 2 Colorimetric Assay Kit, VDVAD
      OverviewActivation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's ICH-1/Caspase-2 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity.

      For research use only; Not for use in diagnostic procedures
      Alternate Names
      • ICH1
      Materials Required but Not Delivered· Microcentrifuge and 1.5 mL Microcentrifuge tubes

      · 37°C water bath or incubator

      · Spectrophotometer

      · 96 well plate
      References
      Product Information
      Components
      • Cell Lysis Buffer - 100 mL
      • 2X Reaction Buffer - 4 x 2 mL.
      • DTT (1 M): 400 μL
      • VDVAD-pNA Substrate: 500 μL
      • Dilution Buffer: 100 mL
      Detection methodChromogenic
      Applications
      ApplicationThe ICH-1/Caspase-2 Colorimetric Protease Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence VDVAD.
      Key Applications
      • Activity Assay
      Biological Information
      Species Reactivity
      • Mammals
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.
      Gene Symbol
      • CASP2
      • NEDD2
      • ICH1
      • NEDD-2
      • ICH-1L/1S
      • CASP-2
      • ICH-1L
      • EC 3.4.22.55 [Contains: Caspase-2 subunit p18
      • Caspase-2 subunit p13
      • Caspase-2 subunit p12].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42575 # Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
      SIZE: 452 amino acids; 50685 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD.
      TISSUE SPECIFICITY: Expressed in larger amounts in the embryonic lung, liver and kidney than in the heart and brain. In the adults higher level expression is seen in the placenta, lung, kidney, pancreas than in the heart, brain, liver and skeletal muscle.
      PTM: The mature protease can process its own propeptide, but not that of other caspases.
      SIMILARITY: SwissProt: P42575 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWe recommend storing reagents at -20°C upon arrival. (Store Cell Lysis Buffer and 2X Reaction Buffer and dilution buffer at 4°C after opening). The performance of this product is guaranteed for 6 months.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation