17-500 Catch and Release® v2.0 Reversible Immunoprecipitation System

17-500
1 kit  50 immunoprecipitations
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey Applications
      AIP, IPK, WB
      Description
      Catalogue Number17-500
      Brand Family Upstate
      Trade Name
      • Catch & Release
      • Upstate
      DescriptionCatch and Release® v2.0 Reversible Immunoprecipitation System
      OverviewFeatures & Benefits:
      Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
      Convenient: spin-column format dramatically simplifies sample handling
      Reversible: simple elution of antigen:antibody complex in either native or denatured form



      Use:
      This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
      References
      Product Information
      Components
      • Catch and Release® Wash Buffer, 10X (Cat.# 20-210)
      • Catch and Release® Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
      • Catch and Release® Denaturing Elution Buffer, 1X (Cat.# 20-284)
      • 20-285
      • 20-216
      • Catch and Release® Capture Tubes
      Applications
      ApplicationCatch & Release v2.0 Reversible Immunoprecipitation System to purify both native & denatured proteins using a variety of antibodies & sample types. Comes in a package of 5 immunoprecipitations.
      Key Applications
      • Immunoprecipitation
      • IP-Kinase Assay
      • Western Blotting
      Application NotesCatch and Release® v2.0 should not be used with proteins containing a 6-His tag.
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoprecipitation.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAll components to be stored at 4°C except the Catch and Release® Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
      Packaging Information
      Material Size1 kit
      Material Package50 immunoprecipitations
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - 21177472117747
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - 21177482117748
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - 20695182069518
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - 21899662189966
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - 22120432212043
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - DAM1447075DAM1447075
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - DAM1478860DAM1478860
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - DAM1597335DAM1597335
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - DAM1718534DAM1718534
      Catch and Release#174; v2.0 - 21397002139700

      References

      Reference overviewApplicationPub Med ID
      A novel radioresistant mechanism of galectin-1 mediated by H-Ras-dependent pathways in cervical cancer cells.
      Huang, E-Y, et al.
      Cell Death Dis, 3: e251 (2012)  2012

      Show Abstract
      22237208 22237208
      Nuclear import of early growth response-1 involves importin-7 and the novel nuclear localization signal serine-proline-serine.
      Jinbiao Chen,Mary Y Liu,Christopher R Parish,Beng H Chong,Levon Khachigian
      The international journal of biochemistry & cell biology  43  2011

      Show Abstract
      21419860 21419860
      p300 Acetyltransferase activity differentially regulates the localization and activity of the FOXO homologues in skeletal muscle.
      Senf, SM; Sandesara, PB; Reed, SA; Judge, AR
      American journal of physiology. Cell physiology  300  C1490-501  2011

      Show Abstract
      21389279 21389279
      INFLUENCE OF GLUCOSAMINE ON GLOMERULAR MESEANGIAL CELL TURNOVER: IMPLICATIONS FOR HYPERGLYCEMIA AND HEXOSAMINE PATHWAY FLUX.
      James LR, Le C, Scholey JW
      Am J Physiol Endocrinol Metab  298  2009

      Show Abstract Full Text Article
      19903862 19903862
      Hypercholesterolemia-induced Abeta accumulation in rabbit brain is associated with alteration in IGF-1 signaling.
      Sharma, Sunita, et al.
      Neurobiol. Dis., 32: 426-32 (2008)  2008

      18775495 18775495
      Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal stem cells on laminin-5.
      Roman M Salasznyk,Robert F Klees,Adele Boskey,George E Plopper
      Journal of cellular biochemistry  100  2007

      Show Abstract
      16927379 16927379
      Focal adhesion kinase signaling pathways regulate the osteogenic differentiation of human mesenchymal stem cells.
      Roman M Salasznyk,Robert F Klees,William A Williams,Adele Boskey,George E Plopper
      Experimental cell research  313  2007

      Show Abstract Full Text Article
      17081517 17081517
      The expression of p63 is associated with the differential stage in nasopharyngeal carcinoma and EBV infection
      Guo, Can, et al
      Journal of translational medicine [electronic resource], 4:23 (2006)  2006

      Immunoprecipitation16729897 16729897
      A-kinase-interacting protein localizes protein kinase A in the nucleus.
      Sastri, Mira, et al.
      Proc. Natl. Acad. Sci. U.S.A., 102: 349-54 (2005)  2005

      Show Abstract
      Immunoprecipitation15630084 15630084
      Elevated vascular endothelial growth factor production in islets improves islet graft vascularization
      Zhang, N., et al
      Diabetes, 53:963-70 (2004)  2004

      Immunoprecipitation15047611 15047611

      Brochure

      Title
      An Introduction to Antibodies and Their Applications

      User Guides

      Title
      Catch and Release v2.0 Reversible Immunoprecipitation System

      Related Products & Applications

      Kit Component

      Catalogue Number Description  
      20-209 Catch and Release® Non-denaturing Elution Buffer, 4X Show Pricing & Availability
      20-210 Catch and Release® Wash Buffer, 10X Show Pricing & Availability
      20-284 Catch and Release® Denaturing Elution Buffer, 1X Show Pricing & Availability

      Product Families

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Immunoprecipitation Kits & Reagents
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Immunoprecipitation Kits & Reagents