|Do the harvest plates replace the MultiScreen MAFB and MAFC plates?||1. No. The design of Harvest plates do not allow for the collection of filtrate or incubation. For assay development, or for any assay where incubation in the filtration plate or where filtrate collection is required must be done in the MultiScreen assay plates.
2. These plates are available with glass fiber filters only. Assays requiring different filter matrices must be done in MultiScreen assay plates.
|How do I seal the wells of a MultiScreen Harvest plate prior to analysis in a 96 well scintillation counter?||1. Seal the bottom of the wells with scintillant -resistant tape (opaque for top reading instruments, Cat. # MATAH0P00; clear for bottom reading or coincidence counting, Cat. # MATAHCL00).
2. Add scintillant to the wells.
3. Seal the top of the plate with clear tape, Cat. # MATAHCL00.
Drug discovery groups that once tested hundreds of novel chemical entities (NCEs) per screen now process thousands daily. This increase in throughput is due in large part to the advances in lead compound generation methods like combinatorial chemistry synthesis. Using this technique, researchers can rapidly produce large compound libraries, which are then screened for interactions with the disease target.
Bead Cleavage Protocol
|1.||Place synthesis beads in suitable reaction vessel|
|2.||Add reactants; seal; react|
|3.||Transfer beads to MultiScreen filter plate; add cleavage chemicals; incubate|
|4.||Filter drug compound into receiver plate, leaving beads in MultiScreen plate|
MultiScreen Solvinert Plates
All MultiScreen Solvinert plate materials are selected for broad chemical compatibility and enable extended in-plate incubations with no leaking. The one-piece plate designs incorporate rigid sidewalls to facilitate use with robotic gripper arms and provide space for barcode labels.
They are available with chemically-resistant hydrophilic and hydrophobic membranes to accommodate aqueous and non-aqueous samples. Both membranes are highly retentive (>99% retention of acid precipitated BSA).
For the broadest chemical resistance, hydrophobic PTFE membrane is recommended. Designed for extended sample incubation times and the lowest extractables, hydrophobic PTFE membrane is ideal for NCE cleavage and cleanup, as well as peptide synthesis. They are also suitable for sample recovery following in-plate compound cleavage from solids or beads.
Hydrophilic PTFE membrane is optimized for low drug and protein binding with excellent throughput in typical aqueous and solvent sample preparation. High sample recoveries and low extractables provide for optimum analysis by HPLC and LC-MS/MS.