DNA Extraction from Agarose Gels

Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin.

Overview

Specifications

Ordering Information

Extraction Kit and Filter Unit Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon Pack Sizeicon Componentsicon
42600Ultrafree-DA Centrifugal Filter Unit 50 Show Pricing & Availability
LSKGEL050Montage Gel Extraction Kit 50
  • includes 50 Ultrafree-DA devices and 500 mL of 50x concentrated modified TAE Buffer
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Reagent Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon Pack Sizeicon
LSKMTAE50Modified TAE Buffer 50x concentration, 500 mL 1 Show Pricing & Availability

Required Equipment: Variable speed centrifuge

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    Documentation

    Technical Info

    Title
    DNA Extraction from Agarose Gels with the Ultrafree-DA Centrifugal Filter Device

    Data Sheet

    Title
    DNA Template Prep and Sequencing Reaction Cleanup
    Montage DNA Gel Extraction Kit

    FAQ

    QuestionAnswer
    Can I use low melting point (LMP) Agarose with the the Montage Gel Extraction Kit?Millipore has not tested the recoveries with LMP Agarose, but because this Agarose is so soft and deformable, the recoveries are not expected to be as high as recoveries observed when using the Montage Gel Extraction Kit with standard Agarose.
    Can I use the Montage Gel Extraction Kit with low melt agarose ( LMP )?No. LMP Agarose will not work in the Montage Gel Extraction Kit. However, for DNA fragments >125 base pairs the Centricon-100 is an excellent choice.
    Can I use the Montage Gel Extraction Kit with DNA which is larger than 1000bp?Yes, the Montage Gel Extraction Kit is recommended for extraction of DNA up to 10,000bp from agarose gel. For high recovery of larger DNA we recommend using the Centrilutor Micro-Electroelutor or using our protocol for recovery of DNA from LMP Agarose with Microcon-100.
    How can I improve recovery when using the Montage Gel Extraction Kit?Best recovery is achieved by working with <1.25% Agarose. Use a single band and trim off any excess gel. Spin at 5,000xG for approximately 10 minutes.
    How do I order a gel nebulizer?Gel nebulizers are a product enhancement included with the Montage Gel Extraction Kit. They increase the recovery of DNA by macerating the Agarose gel, thus facilitating DNA migration from the gel.
    I'm using the Montage Gel Extraction Kit to extract DNA from agarose according to the operating instructions which recommend using TAE buffer. What is the advantage of TAE over TBE ?Since you are extruding the DNA along with buffer from a gel slice, it is important that the electrophoresis buffer is compatible with downstream manipulations such as cloning and DNA sequencing. We found that TBE inhibits DNA sequencing. TAE, on the other hand, is a perfectly good electrophoreses buffer and all the enzymes that we have tested are fully functional in 1X TAE.
    What is the pore size of the filter in the Montage Gel Extraction Kit?The pore size is 0.45u.
    Should I freeze my gel before adding it to the Montage Gel Extraction Kit?The Montage Gel Extraction Kit allows for quick removal of DNA from Agarose without freezing. The only preparation required is to cut and trim the band of interest from the gel. The recovery of DNA is optimized by including the nebulizer, so there is no need for additional gel preparation.
    The operating instructions for the Ultrafree DA recommend making up a modified TAE buffer. Why use modified TAE rather than standard TAE?The modified TAE has 1/10th the final concentration of Na2EDTA of standard TAE. The activity and specificity of many enzymes used in molecular biology is magnesium-dependent. The lower level of EDTA ensures that the activity of enzymes that you may use down stream is not influenced by EDTA lowering the effective concentration of magnesium (i.e., if present, higher levels of EDTA could sequester much of the magnesium). The new Montage Gel Extraction Kit comes with 500mL of 50x stock modifies TAE buffer.
    Can I add more than one band per device?It is not recommended. When the amount of gel increases, the recovery decreases.

    User Guides

    Title
    DNA Gel Extraction Kit
    Ultrafree-DA DNA Extraction From Agarose Gels