APT151 | DNA Ladder Detection Kit

APT151
50 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsDetection Methods
      ACTChromogenic
      Description
      Catalogue NumberAPT151
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDNA Ladder Detection Kit
      OverviewInternucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. Chemicon's Quick Apoptotic DNA Ladder Detection Kit provides a fast and easy means for detection of DNA fragmentation in apoptotic cells. Unlike other similar kits which require 1-2 days to finish the procedure, the new detection method requires less than 90 minutes to prepare DNA in a single tube, without the need for extraction or the use of columns. DNA fragmentation can be easily visualized by agarose gel electrophoreses. The new procedure increases recovery of small fragmented DNA and therefore improves the sensitivity of the assay.
      Materials Required but Not Delivered· Microcentrifuge and 1.5 mL microcentrifuge tubes

      · 55°C water bath or incubator

      · Phosphate buffered Saline solution (PBS) - [10 mM Na2PO4; 138 mM NaCl; 2.7 mM KCl]

      · Absolute ethanol

      · Equipment and reagents for agarose gel electrophoresis and DNA visualization
      References
      Product Information
      Components
      • TE Lysis Buffer - 2 mL.
      • Enzyme A Solution - 0.25 mL.
      • Enzyme B* - 1 vial
      • Ammonium Acetate Solution - 0.25 mL.
      • DNA Suspension Buffer - 2 mL.
      • Note: Dissolve Enzyme B with 275 μL TE Lysis Buffer and mix well before use. The unused Enzyme B solution should be frozen at -70°C.
      Detection methodChromogenic
      Applications
      ApplicationThe Quick Apoptotic DNA Ladder Detection Kit provides a fast & easy means for detection of DNA fragmentation in apoptotic cells.
      Key Applications
      • Activity Assay
      Biological Information
      Species Reactivity
      • Mammals
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWe recommend storing reagents at -20°C upon arrival. Frequent freeze-thawing is not recommended. The performance of this product is guaranteed for 6 months.
      Packaging Information
      Material Size50 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overviewPub Med ID
      Identification of a novel antiapoptotic human protein kinase C delta isoform, PKCdeltaVIII in NT2 cells.
      Kun Jiang, André H Apostolatos, Tomar Ghansah, James E Watson, Timothy Vickers, Denise R Cooper, P K Epling-Burnette, Niketa A Patel
      Biochemistry  47  787-97  2008

      Show Abstract
      18092819 18092819

      User Guides

      Title
      DNA Ladder Detection Kit