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Description
Overview
A hydrazinecarbothioamide derived intramimic that acts as an agonist of mammalian Diaphanous (mDia)-related formins and disrupts diaphanous inhibitory domain (DID) and diaphanous autoregulatory domain (DAD) interaction (IC50 = 140 nM). Shown to trigger actin assembly and stabilize microtubules in a dose -dependent manner and at levels similar to those induced by Taxol. Induces caspase-3 activation and apoptosis in NIH 3T3 and SW480 cells(~ 100 µM) and slows the growth of SW480 xenograft tumors in mice (~ 25 mg/kg). Shown to induce LacZ expression, producing b-gal activity that is comparable to effects seen with cytochalasin D treatment.
Please note that the molecular weight for this compound is batch-specific due to variable water content.
Catalogue Number
509583
Brand Family
Calbiochem®
Synonyms
Formin Activator
References
References
Lash, L.L., et al. 2013. Cancer Res.73, 6793. Copeland, S.J. et al. 2007. J Biol. Chem. 282, 30120.
Product Information
Form
Off-white powder
Hill Formula
C₁₂H₁₇N₃O₂S
Chemical formula
C₁₂H₁₇N₃O₂S
Reversible
Y
Structure formula Image
Applications
Biological Information
Primary Target
mDia
Primary Target IC<sub>50</sub>
140 nM
Purity
≥98% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity
Standard Handling
Storage
+2°C to +8°C
Protect from Light
Protect from light
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Lash, L.L., et al. 2013. Cancer Res.73, 6793. Copeland, S.J. et al. 2007. J Biol. Chem. 282, 30120.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
14-March-2014 JSW
Synonyms
Formin Activator
Description
A hydrazinecarbothioamide derived intramimic that acts as an agonist of mammalian Diaphanous (mDia)-related formins and disrupts diaphanous inhibitory domain (DID) and diaphanous autoregulatory domain (DAD) interaction (IC50 = 140 nM). Shown to trigger actin assembly and stabilize microtubules in a dose -dependent manner and at levels similar to those induced by Taxol. Induces caspase-3 activation and apoptosis in NIH 3T3 and SW480 cells(~ 100 µM) and slows the growth of SW480 xenograft tumors in mice (~ 25 mg/kg). Shown to induce LacZ expression, producing b-gal activity that is comparable to effects seen with cytochalasin D treatment.
Form
Off-white powder
Intert gas (Yes/No)
Packaged under inert gas
Chemical formula
C₁₂H₁₇N₃O₂S
Structure formula
Purity
≥98% by HPLC
Solubility
DMSO (100 mg/ml)
Storage
+2°C to +8°C Protect from light
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Lash, L.L., et al. 2013. Cancer Res.73, 6793. Copeland, S.J. et al. 2007. J Biol. Chem. 282, 30120.