ECM540 | ECM Cell Adhesion Array Kit, colorimetric

ECM540
1 plate  96 wells
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      Overview

      Replacement Information

      Key Spec Table

      Detection Methods
      Chromogenic
      Description
      Catalogue NumberECM540
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionECM Cell Adhesion Array Kit, colorimetric
      OverviewIntroduction:

      Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion, migration, and invasion of many diverse cell types on various extracellular matrix (ECM) component proteins. The CHEMICON® ECM Cell Adhesion Array Kit (Colorimetric) provides an ECM Array microtiter plate with a colorimetric detection format, allowing for large-scale screening and quantitative comparison of multiple samples/cell lines. The kit is designed as a rapid (1-2 hours), cost effective, efficient method for characterization of cell adhesion.

      In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

      · Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530)

      · Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531)

      · Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532)

      · Alpha Integrin-Mediated Cell Adhesion Array Kit (Fluorimetric) (ECM533)

      · Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorimetric) (ECM534)

      · Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Fluorimetric) (ECM535)

      · ECM Cell Adhesion Array Kit (Fluorimetric) (ECM545)

      · QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)

      · QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)

      · QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)

      · QCM™ 96-well Cell Invasion Assay (ECM555)

      · QCM™ 96-well Collagen-Based Cell Invasion Assay (ECM 556)

      · 24-well Insert Cell Migration and Invasion assay systems

      · CytoMatrix™ Cell Adhesion strips (ECM protein coated)

      · QuantiMatrix™ ECM protein ELISA kits



      Test Principle:

      The CHEMICON® ECM Cell Adhesion Array kit utilizes a colorimetric detection format, allowing large-scale screening and quantitative comparison of multiple samples. Each kit contains a 96-well, microtiter plate consisting of 12 X 8-well removable strips. Each well within a strip (7 wells total) is pre-coated with a different ECM protein (see table below) along with one BSA-coated well (negative control). Experimental cells are seeded onto the coated substrates, where adherent cells are captured. Subsequently, unbound cells are washed away, and the adherent cells are fixed and stained. After stain extraction, the relative cell attachment is determined using absorbance readings.



      Additional information about the ECM components provided in the kit:
      Materials Required but Not Delivered· Multi-channel and/or repeating pipettes.

      · Harvesting buffer: EDTA or trypsin cell detachment buffer. Suggested formulations include a) 2 mM EDTA/PBS, b) 0.05% trypsin in Hanks Balanced Salt Solution (HBSS) containing 25 mM HEPES, or other cell detachment formulations as optimized by individual investigators.

      Note: Trypsin cell detachment buffer maybe required for difficult cell lines. Allow sufficient time for cell receptor recovery.

      · Tissue culture growth medium appropriate for subject cells, such as DMEM containing 10% FBS.

      · Quenching Medium: serum-free medium, such as DMEM, EMEM, or FBM (fibroblast basal media), containing 5% BSA.

      Note: Quenching Medium must contain divalent cations (Mg2+, Ca2+) sufficient for quenching EDTA in the harvesting buffer.

      · Sterile PBS or HBSS to wash cells.

      · Deionized water.

      · Low speed centrifuge and tubes for cell harvesting.

      · CO2 incubator appropriate for subject cells.

      · Sterile cell culture hood.

      · Hemacytometer and microscope for counting cells.

      · Trypan Blue or equivalent viability stain.

      · Microplate reader (540-570 nm detection) or spectrophotometer
      References
      Product Information
      Components
      • ECM Array Plate: (PN: 90652) One 96-well plate with 12 strips. Each 8-well strip consists of 7 different human, ECM protein-coated wells (Collagen I, Collagen II, Collagen IV, Fibronectin, Laminin, Tenascin, Vitronectin) and one BSA-coated well (negative control). See the plate layout on Data sheet.
      • Cell Stain Solution: (Part No. 90144) One bottle - 20 mL.
      • Extraction Buffer: (Part No. 90145) One bottle - 20 mL.
      • Assay Buffer: (Part No. 90601) One bottle - 100 mL.
      Detection methodChromogenic
      Applications
      ApplicationThe ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.
      Application NotesThe CHEMICON® ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.



      For Research Use Only. Not for use in diagnostic procedures.
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe ECM Array Plate can be stored at 2° to 8°C in the foil pouch up to its expiration date. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed. Keep the remaining kit components at 2° to 8°C.

      Precautions:

      · Cell stain contains minor amounts of crystal violet, a toxic substance, which may cause cancer and heritable genetic damage. Handle with caution. Toxic by inhalation and if swallowed. Irritating to eyes, respiratory system and skin.

      · Extraction buffer contains alcohol. Avoid internal consumption.
      Packaging Information
      Material Size1 plate
      Material Package96 wells
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Analysis of the disintegrin-metalloproteinases family reveals ADAM29 and ADAM7 are often mutated in melanoma.
      Xiaomu Wei,Angela Moncada-Pazos,Santiago Cal,Clara Soria-Valles,Jared Gartner,Udo Rudloff,Jimmy C Lin, ,Steven A Rosenberg,Carlos López-Otín,Yardena Samuels,Carlos López-Otín
      Human mutation  32  2011

      Show Abstract
      21618342 21618342

      Technical Info

      Title
      Integrin Profiling of Stem Cells and Differentiated Progeny
      Screening Kits to Monitor Cell - Extracellular Matrix Interactions

      Data Sheet

      Title
      ECM Cell Adhesion Array Kit, colorimetric - Data Sheet
      ECM Cell Adhesion Array Kit, colorimetric - Data Sheet

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Adhesion Assays