ECM545 | ECM Cell Adhesion Array Kit, fluorometric

ECM545
1 plate  96 wells
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      Overview

      Replacement Information

      Key Spec Table

      Detection Methods
      Fluorescent
      Description
      Catalogue NumberECM545
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionECM Cell Adhesion Array Kit, fluorometric
      OverviewIntroduction:

      Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion, migration, and invasion of many diverse cell types on various extracellular matrix (ECM) component proteins. The CHEMICON® ECM Cell Adhesion Array Kit (Fluorometric) provides an ECM Array microtiter plate with a homogenous fluorescence detection format, allowing for large-scale screening and quantitative comparison of multiple samples/cell lines. The kit is designed as a rapid (1-2 hours), cost effective, efficient method for characterization of cell adhesion.

      In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

      · Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530)

      · Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531)

      · Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532)

      · Alpha Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM533)

      · Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM534)

      · Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Fluorometric) (ECM535)

      · ECM Cell Adhesion Array Kit (Colorimetric) (ECM540)

      · QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)

      · QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)

      · QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)

      · QCM™ 96-well Cell Invasion Assay (ECM555)

      · QCM™ 96-well Collagen-Based Cell Invasion Assay (ECM 556)

      · 24-well Insert Cell Migration and Invasion assay systems

      · CytoMatrix™ Cell Adhesion strips (ECM protein coated)

      · QuantiMatrix™ ECM protein ELISA kits



      Test Principle:

      The CHEMICON® ECM Cell Adhesion Array kit utilizes a homogenous fluorescence detection format, allowing large-scale screening and quantitative comparison of multiple samples. Each kit contains a 96-well, microtiter plate consisting of 12 X 8-well removable strips. Each well within a strip (7 wells total) is pre-coated with a different ECM protein (see table below) along with one BSA-coated well (negative control). Experimental cells are seeded onto the coated substrates, where adherent cells are captured. Subsequently, unbound cells are washed away, and the adherent cells are lysed and detected by the patented CyQuant GR® dye (Molecular Probes) (1-2). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (3). Relative cell attachment is determined using a fluorescence plate reader.

      Additional information about the ECM components provided in the kit:
      Materials Required but Not Delivered· Multi-channel and/or repeating pipettes.

      · Harvesting buffer: EDTA or trypsin cell detachment buffer. Suggested formulations include a) 2 mM EDTA/PBS, b) 0.05% trypsin in Hanks Balanced Salt Solution (HBSS) containing 25 mM HEPES, or other cell detachment formulations as optimized by individual investigators.

      Note: Trypsin cell detachment buffer maybe required for difficult cell lines. Allow sufficient time for cell receptor recovery.

      · Tissue culture growth medium appropriate for subject cells, such as DMEM containing 10% FBS.

      · Quenching Medium: serum-free medium, such as DMEM, EMEM, or FBM (fibroblast basal media), containing 5% BSA.

      Note: Quenching Medium must contain divalent cations (Mg2+, Ca2+) sufficient for quenching EDTA in the harvesting buffer.

      · Sterile PBS or HBSS to wash cells.

      · Deionized water.

      · Low speed centrifuge and tubes for cell harvesting.

      · CO2 incubator appropriate for subject cells.

      · Sterile cell culture hood.

      · Hemacytometer and microscope for counting cells.

      · Trypan Blue or equivalent viability stain.

      · Fluorescence plate reader with 485nm and 530nm filter set.

      · 96-well plate suitable for fluorescence measurement.
      References
      Product Information
      Components
      • ECM Array Plate: (PN: 90652) One 96-well plate with 12 strips. Each 8-well strip consists of 7 different human, ECM protein-coated wells (Collagen I, Collagen II, Collagen IV, Fibronectin, Laminin, Tenascin, Vitronectin) and one BSA-coated well (negative control). See the plate layout on data sheet.
      • 4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.
      • CyQuant GR® Dye: (Part No. 90132) One vial - 75 μL.
      • Assay Buffer: (Part No. 90601) One bottle - 100 mL.
      Detection methodFluorescent
      Applications
      ApplicationThe ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.
      Application NotesThe ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.

      For Research Use Only. Not for use in diagnostic procedures.
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe ECM Array Plate can be stored at 2° to 8°C in the foil pouch up to its expiration date. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed. Keep the remaining kit components at 2° to 8°C.

      Precautions:

      No data is available on the biological toxicity of CyQuant GRâ dye. This reagent binds to nucleic acids, and as such, should be treated as a potential mutagen which may cause cancer and heritable genetic damage. Handle with caution. The DMSO stock solution should be handled with special caution as DMSO can facilitate the entry of organic molecules into tissues.
      Packaging Information
      Material Size1 plate
      Material Package96 wells
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Transmembrane motility assay of transiently transfected cells by fluorescent cell counting and luciferase measurement.
      Gildea, J J, et al.
      BioTechniques, 29: 81-6 (2000)  2000

      Show Abstract
      10907081 10907081

      Technical Info

      Title
      Integrin Profiling of Stem Cells and Differentiated Progeny
      Screening Kits to Monitor Cell - Extracellular Matrix Interactions

      Data Sheet

      Title
      ECM Cell Adhesion Array Kit, fluorimetric - Data Sheet

      User Guides

      Title
      ECM Cell Adhesion Array Kit (Fluorimetric)

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Adhesion Assays