115934 | Eosin B (bluish) (C.I.45400)

115934
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      Overview

      Replacement Information

      Key Spec Table

      CAS #EC NumberHill FormulaMolar Mass
      548-24-3208-943-1C₂₀H₆Br₂N₂Na₂O₉624.06 g/mol

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      1159340025
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          Glass bottle 25 g
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          1159340100
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              Glass bottle 100 g
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              Description
              Catalogue Number115934
              SynonymsDibromodinitrofluorescein sodium
              DescriptionEOSIN B (C.I. 45400) FOR MICROSCOPY CERT
              OverviewEosin B (C.I. 45400) is a CERTISTAIN® dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin B staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances red-orange.
              As the dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed.
              References
              Product Information
              CAS number548-24-3
              EC number208-943-1
              Hill FormulaC₂₀H₆Br₂N₂Na₂O₉
              Molar Mass624.06 g/mol
              HS Code3204 12 00
              Structure formula Image
              Applications
              ApplicationEosin B (bluish) (C.I.45400) for microscopy Certistain®. CAS 548-24-3, pH 6.2 (10 g/l, H₂O, 25 °C).
              Biological Information
              Physicochemical Information
              pH value6.2 (10 g/l, H₂O, 25 °C)
              Bulk density680 kg/m3
              Solubility300 g/l
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Storage class10 - 13 Other liquids and solids
              WGKWGK 2 water endangering
              Disposal3
              Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              StorageStore at +5°C to +30°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Dye content (gravimetrically)≥ 85 %
              Identity
              - sodium

              passes test
              - UV/VIS-Spectrumpasses test
              Absorption maximum λ max (water)517 - 525 nm
              Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water)800 - 1000
              TLC-Testpasses test
              Loss on drying (110 °C)≤ 8 %
              Suitability for microscopypasses test

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              Product Number PackagingSpecificationLot Number
              1159340025 Glass bottlePDF Specification Document
              1159340100 Glass bottlePDF Specification Document

              Brochure

              Title
              Alles für die Histologie. Schnell und zuverlässig.
              Products for microscopy - All you need for successful working

              Reach Use

              Title
              Reach Use

              Instructions for Use

              Title
              115934 Eosin B (bluish) (C.I. 45400) for microscopy Certistain®
              115934 Eosin B (bläulich) (C.I. 45400) für die Mikroskopie Certistain®
              115934 Eosina A (azzurroguolo) (C.I. 45400) per microscopia Certistain®
              115934 Eosina B (azulada) (C.I. 45400) para microscopía Certistain®
              115934 Eosine B (bleuâtre) (C.I. 45400) pour la microscopie Certistain®

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              Accessories (Primary)

              Catalogue Number Description  
              115935 Eosin Y (yellowish) (C.I. 45380) Show Pricing & Availability

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              Categories

              IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Certistain® Dyes
              Reagents, Chemicals and Labware > Inorganic Reagents > Safety Products and General Applications > Indicators > Acid Alkali Indicators or pH Indicators