FTAV00003 | Fast Trap Adenovirus Purification and Concentration Kit

3 purifications and concentrations  3 purifications and concentrations
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      Replacement Information
      Catalogue NumberFTAV00003
      Trade Name
      • Fast-Trap
      DescriptionFast Trap Adenovirus Purification and Concentration Kit
      OverviewThe Fast-Trap Adenovirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification of ≥ 3 mL of adenovirus serotype 5, up to a capacity of approximately 1x1013 total adenovirus particles.

      The purification kit protocol calls for releasing the virus from the infected cells using multiple freeze-thaw cycles, then collecting the crude adenovirus. Most of the large cellular debris is removed by centrifugation, leaving the viable virus particles in the supernatant. The supernatant is further clarified by passing it through a Steriflip-HV 0.45 μm filter. After adding a binding buffer, the virus solution is passed over a treated filter that adsorbs the virus particles, allowing the smaller cellular debris and media to pass through the filter. Following a wash to remove any weakly bound proteins, the virus is eluted off the filter with an elution buffer. Finally, the virus can be concentrated and exchanged into the desired buffer using an Amicon® Ultra centrifugal filter unit.

      For laboratory use only; not for diagnostic or in vivo use.
      Background InformationMillipore’s Fast-Trap Adenovirus and Lentivirus Purification and Concentration Kits provide a fast, safe, and easy alternative for viral purification. The kits contain the necessary components to accommodate the entire virus purification workflow. The purification results in high recovery of viable viral particles with good purity.

      Fast-Trap Advantages
      - Saves time: protocol can be completed in as little as two hours
      - Higher yield: recover up to 70% of viral particles
      - Dependable: simple protocol ensures quality results
      - Easy: Steriflip filters eliminate messy purification steps and expensive equipment
      - Safe: close vacuum device eliminate potential spills

      In one experiment, we tested Millipore’s Fast-Trap kit with other common methods to determine which methods gave the best recoveries in the least time. The Fast-Trap Kit was faster than the other methods while giving similar recovery.
      Product Information
      • 3 – Fast-Trap virus filter units
        3 – Steriflip-HV filter units
        1 – 10x Binding buffer, 20 mL
        1 – Equilibration buffer, 100 mL
        1 – Wash buffer, 200 mL
        1 – Elution buffer, 21 mL
        3 – Amicon Ultra-4 filter units, 50 kDa NMWL
        1 – Test tube stand
        1 – User Guide
        1 – Overview of Procedure card
        1 – Material Safety Data Sheet
      Key Applications
      • Adenovirus Purification and Concentration
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • General Lab Use
      Storage and Shipping Information
      Storage ConditionsThe Fast-Trap Adenovirus Purification and Concentration Kit should be stored at room temperature.

      Warnings and Precautions:
      WARNING: This kit permits quick purification of adenovirus, an infectious agent which should be handled under BSL2 safety precautions. Wear hand, eye, face and body personal protective
      equipment (PPE) when using this kit. Millipore Corporation takes no responsibility for improper use of this kit.

      WARNING:All virus purification steps (except water bath incubation and centrifugation) should be conducted in a biosafety cabinet.
      For research use only. Not for use in diagnostic or human clinical procedures.
      Variation in the amount of adenovirus purified with this kit may be due in part to infection success and cell conditions.
      Packaging Information
      Material Size3 purifications and concentrations
      Material Package3 purifications and concentrations
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Technical Info

      Improved Method for Adenovirus and Lentivirus Purification using the Fast-Trap™ Virus Purification and Concentration Kit

      Data Sheet

      Fast-Trap™- Lentivirus and Adenovirus Purification and Concentration Kits


      A New Vacuum-Based Method for Adenovirus Purification and Concentration
      New Workflow for Lentivirus Purification, Concentration, and Immunodetection,
      Poster: Improved Method For Adeno and Lenti- Virus Purification and Titration
      Poster: Improved Method for Adeno and Lenti-Virus Purification and Titration

      User Guides

      Fast-Trap Adenovirus Purification and Concentration Kit
      Fast-Trap Adenovirus Purification and Concentration Kit