FCCH100106 | FlowCellect MitoDamage Kit

FCCH100106
100 Tests  
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      Overview

      Replacement Information
      Description
      Catalogue NumberFCCH100106
      Trade Name
      • FlowCellect
      DescriptionFlowCellect MitoDamage Kit
      OverviewMillipore’s FlowCellect™MitoDamage Kit includes MitoSense Red, a fluorescent cationic dye that accumulates in the mitochondria, and is responsive to mitochondrial potential changes. Annexin V conjugated to a green sensitive dye, CF488A, which binds to phosphatidylserine (PS) on the surface of apoptotic cells. And the cell impermeant DNA intercalator 7-Aminoactinomycin-D (7-AAD), a dead cell dye. The simultaneous use of the reagents allows researchers to obtain information on early, mid and late apoptosis in one simple assay.
      MitoSense Red is excitable by a red laser and fluoresces maximally at 650 nm (Red2 fluorescence on the guava easyCyte 8HT). Uninduced cells with intact mitochondrial membrane potential demonstrate high Red2 fluorescence while cells which have impaired mitochondrial membrane potential depict lower Red2 fluorescence. Annexin V is calcium-dependent phospholipid binding protein with high affinity for PS, a membrane component normally localized to the internal face of the cell membrane. It is conjugated to CF488A which is excited by a 488 nm laser and emits at 525 nm. Control cells depict no green fluorescence while apoptotic cells exhibit positive green fluorescence. The 7-AAD dye included in the kit monitors cell membrane permeability changes typically observed later in apoptosis as well as necrotic cell death. 7-AAD is excluded from live, healthy cells as well as early apoptotic cells and these cells have low red fluorescence. The kit can thus distinguish multiple populations: 1) Live cells with intact mitochondrial membrane 2) Cells with dissipated membrane potential but no Annexin V or 7AAD staining 3) Early apoptotic cells with dissipated membrane potential and Annexin V binding 4) Late Apoptotic Cells or dead cells with dissipated membrane potentials. The kit thereby provides a complete picture of mitochondrial health and cell health and allows the correlation of mitochondrial damage to cell health. The entire assay can be performed in less than30 minutes once cellular samples are ready.
      Alternate Names
      • FlowCellect
      References
      Product Information
      Components
      • MitoSense Red Dye
      • Annexin CF 488
      • 7-AAD
      • 10X Assay Buffer
      • User Manual
      Detection methodFluorescent
      Instrument compatibility
      • EasyCyte 8HT
      Applications
      ApplicationThis FlowCellectMitoDamage Kit for Flow Cytometry includes MitoSense Red, a fluorescent cationic dye that accumulates in the mitochondria & is responsive to mitochondrial potential changes.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size100 Tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      FlowCellect MitoDamage Kit - 12-0165 12-0165
      FlowCellect MitoDamage Kit - 12-0308 12-0308
      FlowCellect MitoDamage Kit - 12-0469 12-0469
      FlowCellect MitoDamage Kit - 13-0214 13-0214
      FlowCellect MitoDamage Kit - 13-0550 13-0550
      FlowCellect MitoDamage Kit - 14-0061 14-0061
      FlowCellect MitoDamage Kit - 14-0100 14-0100
      FlowCellect MitoDamage Kit - 14-0162 14-0162
      FlowCellect® MitoDamage Kit 4600-3287MAN

      Brochure

      Title
      Benchtop Flow Cytometry Solutions Kits and Reagents
      Flow cytometry solutions for your cell health experiments.
      Innovative Solutions for Flow Cytometry Analysis

      Data Sheet

      Title
      Track Cell Health With NovaQUANT™ Mitochondrial qPCR Panels

      User Guides

      Title
      FlowCellect™ MitoDamage Kit (100 Tests)

      Related Products & Applications

      Software Compatibility

      Catalogue Number Description  
      0500-4120 InCyte Software for Guava® easyCyte HT Systems Show Pricing & Availability

      Product Families

      Related Products By: Brand Facete

      Categories

      Life Science Research > Cell Analysis > guava easyCyte Flow Cytometers > Flow Cytometry Kits & Reagents