FCCH100105 | FlowCellect MitoPotential Red Kit

FCCH100105
100 Tests  
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      Overview

      Replacement Information
      Description
      Catalogue NumberFCCH100105
      Trade Name
      • FlowCellect
      DescriptionFlowCellect MitoPotential Red Kit
      OverviewMillipore’s FlowCellect™ MitoPotential Red Kit is a dual parameter assay kit that includes MitoSense Red , a fluorescent cationic dye that accumulates in the mitochondria and is responsive to mitochondrial potential changes, and the DNA intercalator 7-Aminoactinomycin-D (7-AAD), a membrane impermeant dead cell dye. The simultaneous use of the reagents allows researchers to obtain information on early and late apoptosis in one simple assay. MitoSense Red is excitable by a red laser and fluoresces maximally at 650 nm (Red2 fluorescence on the guava easyCyte 8HT). Uninduced cells with intact mitochondrial membrane potential demonstrate high Red2 fluorescence while cells which have impaired mitochondrial membrane potential depict lower Red2 fluorescence. The 7-AAD dye included in the kit monitors cell membrane permeability changes typically observed later in apoptosis as well as necrotic cell death. 7-AAD is excluded from live, healthy cells as well as early apoptotic cells, which have low red fluorescence. The kit can thus distinguish 3 populations: 1) Live cells with intact mitochondrial membrane 2) Early apoptotic cells with dissipated membrane potential but no 7AAD staining 3) Late Apoptotic Cells or dead cells that have dissipated membrane potential and compromised membranes demonstrate an increase in red fluorescence. The entire assay can be performed in approximately 30 min once cellular samples are ready. Sufficient reagents are provided for 100 tests. The kit includes optimized preformulated dyes and buffers necessary for cell preparation and analysis on a flow cytometer equipped with a blue and red laser.
      The FlowCellect™ MitoPotential Red Kit involves simple and fairly rapid assay protocols, requires no compensation and allows for easy interpretation of test results.
      Alternate Names
      • FlowCellect
      References
      Product Information
      Components
      • MitoSense Red Dye
      • 7-AAD
      • 10X Assay Buffer HSC
      • User Manual
      Detection methodFluorescent
      Instrument compatibility
      • EasyCyte 8HT
      Applications
      ApplicationThis FlowCellect MitoPotential Red Kit for Flow Cytometry is a dual parameter assay Kit for Flow Cytometry that includes MitoSense Red, a fluorescent cationic dye & the DNA intercalator 7-Aminoactinomycin-D (7-AAD).
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size100 Tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      FlowCellect MitoPotential Red Kit - 10-0275 10-0275
      FlowCellect MitoPotential Red Kit - 10-0492 10-0492
      FlowCellect MitoPotential Red Kit - 10-0555 10-0555
      FlowCellect MitoPotential Red Kit - 12-0164 12-0164
      FlowCellect MitoPotential Red Kit - 12-0466 12-0466
      FlowCellect MitoPotential Red Kit - 13-0211 13-0211
      FlowCellect MitoPotential Red Kit - 13-0294 13-0294
      FlowCellect MitoPotential Red Kit - 13-0549 13-0549
      FlowCellect MitoPotential Red Kit - 13-0611 13-0611
      FlowCellect MitoPotential Red Kit - 13-0613 13-0613

      Brochure

      Title
      Benchtop Flow Cytometry Solutions Kits and Reagents
      Comprehensive solutions for studying cell health - Life, death, and everything in between.
      Flow cytometry solutions for your cell health experiments.
      Innovative Solutions for Flow Cytometry Analysis

      Data Sheet

      Title
      Track Cell Health With NovaQUANT™ Mitochondrial qPCR Panels

      User Guides

      Title
      FlowCellect MitoPotential Red Kit, 100 Tests

      Newsletters / Publications

      Title
      Cellutions Newsletter: 2011, Vol. 2

      Related Products & Applications

      Software Compatibility

      Catalogue Number Description  
      0500-4120 InCyte Software for Guava® easyCyte HT Systems Show Pricing & Availability

      Product Families

      Related Products By: Brand Facete

      Categories

      Life Science Research > Cell Analysis > guava easyCyte Flow Cytometers > Flow Cytometry Kits & Reagents