FCIM025161 | FlowCellect™ Mouse Th1 Differentiation Tool Kit

FCIM025161
12 Tests / Kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberFCIM025161
      Trade Name
      • FlowCellect
      DescriptionFlowCellect™ Mouse Th1 Differentiation Tool Kit
      OverviewThe FlowCellect Mouse™ Th1 Differentiation Tool Kit includes a CD3 Coated Activation Plate, complete Differentiation Media and Activator/Re-Stimulation Reagents. Detailed assay instructions are included to assist in three culture steps: Activation, Expansion and Re-Stimulation to complete Th1 cell generation in 6 days.

      Day 1=Activation: Culture CD4+ cells with growth factors and TCR co-stimulator on an anti-CD3 coated 96-well plate for 3 days.
      Day 4=Expansion: Expand activated/developing Th1 cells in a larger tissue culture plate.
      Day 6=Re-Stimulation: Differentiated Th1 cells are re-stimulated with mitogens to induce Th1 signature cytokine production on day 6.
      Alternate Names
      • Flow Cellect
      Background InformationCD4+ T cells play a central role in the function of the immune system. They help B cells produce antibodies, enhance and maintain responses of CD8+ T cells, regulate macrophage function, orchestrate immune responses against a wide variety of pathogenic microorganisms, and regulate immune responses.

      Effector T cells are derived from naïve CD4+ progenitor T cells, after maturational complex interactions with antigen-presenting cells in a permissive milieu, including antigenic type and load, and cytokine signaling. Committed CD4+ T cells can differentiate into four major lineages: Th1, Th2, Th17 and regulatory T cells with distinct cytokine products and biological functions.

      The primary role of the Th1 subtype is to address intracellular pathogens, by producing IFNgamma (IFNg), interleukin (IL) -2 and tumor necrosis factor alpha/beta to help cellular immunity. The differentiation to the Th1 lineage is promoted through a series of complex signaling events such as IL-12 which is mainly produced by macrophages as well as IFNg which produced by NK, CD8 and Th1 cells. In addition, neutralizing antibody-mediated IL-4 depletion enhances the generation of Th1 cells in vitro, suggesting that IL-4 which is signature cytokine for Th2 lineage, antagonizes Th1 polarization.
      References
      Product Information
      Components
      • 1. Mouse CD3 Coated Activation Plate: (Part No. CS206556) One 96-well plate with strips.
      • 2. Th1 Activator #1: (Part No. CS206522) One vial containing 33 µL of neutralizing antibodies.
      • 3. Differentiation Media: (Part No. CS206524) One vial containing 200 mL of complete Th1 differentiation culture media
      • 4. Th1 Activator #2: (Part No.CS206523) One vial containing lyophilized growth factors
      • 5. Re-Stimulation Reagent: (Part No.CS206520) One vial containing 48 μL cocktail of PMA and ionomycin in DMSO
      Applications
      ApplicationThe FlowCellect Mouse Th1 Differentiation Tool Kit for Flow Cytometry includes a CD3 Coated Activation Plate, complete Differentiation Media & Activator/Re-Stimulation Reagents.
      Key Applications
      • Flow Cytometry
      Biological Information
      HostMixed Species
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceLot to lot in-process testing on all kit components
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions4-8°C for antibodies and buffers
      Packaging Information
      Material Size12 Tests / Kit
      Transport Information
      Supplemental Information
      Specifications