FCIM025168 | FlowCellect™ Mouse Viable Treg Characterization Kit

FCIM025168
25 Tests / Kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberFCIM025168
      Trade Name
      • FlowCellect
      DescriptionFlowCellect™ Mouse Viable Treg Characterization Kit
      OverviewMillipore’s FlowCellect™ Mouse Viable Treg Characterization kit includes three directly conjugated antibodies, Anti-CD4-PerCP/Cy5.5, Anti-CD25-PE, and Anti-Foxp3-Alexa Fluor® 488. A viability dye is also included to allow for the discrimination of only viable cells during sample analysis. This four color kit is designed to characterize viable Tregs within mixed cell populations.

      Eliminating false results due to non-specific binding of antibodies to dead cells allows researchers to more accurately identify regulatory T-cells from different sample collections regardless of changes in viability between samples. By doing so, Tregs can now be analyzed across multiple samples with a normalized and accurate measurement of their expression levels of each of the antibodies in the kit.

      The antibodies provided have been carefully titrated to ensure the ability to accurately measure the expression of all markers simultaneously. Sufficient reagents are provided to perform 25 four-color tests. Detailed assay instructions are included to assist in analysis and to ensure the correct cell concentration is obtained during acquisition of sample data.
      Alternate Names
      • Flow Cellect
      Background InformationRegulatory T cells (Tregs) are a subpopulation of CD4+ T cells which constitutively express CD25 (IL-2 receptor alpha chain) and the transcription factor Foxp3. Characterization of these cells can be accomplished by performing multi-parameter flow cytometry to identify key combinations of the aforementioned surface and intracellular markers.

      Tregs play a vital role in regulating the responses of the immune system. They suppress the activation of the immune system following infections, which helps maintain immune homeostasis. It has also been shown that some cancer patients have increased numbers of Tregs which may allow some malignant cells to escape the activation of the immune system due to the suppressive nature of the Tregs. Researchers are interested in the mechanism by which cancers are evading anti-tumor immune responses as these could aid in the design of alternate treatment methods.

      A second and equally important function of Treg is promoting tolerance to self-antigens. Tregs act to ensure that self-antigens presented to the immune system do not illicit an immune response. This is yet another way that Tregs help to maintain immune homeostasis by actively suppressing the activation and expansion of self-reactive T cells.
      References
      Product Information
      Components
      • 1. 20X Anti-CD4-PerCP/Cy5.5: (Part No. CS206550) 1 vial containing 150 µL.
      • 2. 20X Anti-CD25-PE: (Part No. CS206540) One vial containing 150 µL antibody.
      • 3. 20X Anti-Foxp3-Alexa Fluor® 488: (Part No. CS206539) One vial containing 150 µL antibody.
      • 4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
      • 5. 4X Fixation/Permeabilization Buffer: (Part No. CS206472) One bottle containing 3.2 mL buffer.
      • 6. Fixation/Permeabilization Diluent: (Part No. CS206473) One bottle containing 9.5 mL buffer.
      • 7. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
      • 8. 10X Permeabilization Buffer: (Part No. CS206474) One bottle containing 6.5 mL buffer
      • 9. 1000X Fixable Viability Dye eFluor®660: (Part No.CF200098) One vial containing 50 µL of dye.
      Detection methodFluorescent
      Applications
      ApplicationThis FlowCellect Mouse Viable Treg Characterization Kit for Flow Cytometry includes three directly conjugated antibodies, Anti-CD4-PerCP/Cy5.5, Anti-CD25-PE & Anti-Foxp3-Alexa Fluor 488.
      Key Applications
      • Flow Cytometry
      Biological Information
      HostMixed Species
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceLot to lot in-process testing on all kit components
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions4-8°C for antibodies and buffers
      Packaging Information
      Material Size25 Tests / Kit
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Software Compatibility

      Catalogue Number Description  
      0500-4120 InCyte Software for Guava® easyCyte HT Systems Show Pricing & Availability

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      Categories

      Life Science Research > Cell Analysis > guava easyCyte Flow Cytometers > Flow Cytometry Kits & Reagents