FCCS025132 | FlowCellect™ p38 Stress Pathway Activation Detection Kit

25 Tests / Kit  
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      Replacement Information
      Catalogue NumberFCCS025132
      Trade Name
      • FlowCellect
      DescriptionFlowCellect™ p38 Stress Pathway Activation Detection Kit
      OverviewMillipore’s FlowCellect™ p38 Stress Pathway Activation Detection Kit is designed to enable a researcher a quick and easy way to detect the phosphorylation state of P38α and ATF2 by flow cytometry. Millipore’s FlowCellect™ p38 Stress Pathway Activation Kit was developed and tested using the activating reagent, anisomycin, in Jurkat cells but the kit can be used with other human cell lines to determine the effect of stress stimuli and chemical reagents that can induce P38 pathway signaling.
      Millipore’s FlowCellect™ p38 Stress Pathway Activation Detection Kit contains sufficient reagents for 25 2-color tests. The kit includes two optimized fluorescent labeled antibodies and buffers necessary for cell preparation and analysis. Detailed assay instructions are included to assist in your analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.
      Alternate Names
      • Flow Cellect
      Background Informationp38 is a member of the mitogen activated kinase family that responds to stress stimuli, such as inflammatory cytokines, ultraviolet irradiation, heat shock, and osmotic shock. p38 is involved in cell differentiation and apoptosis. More recently, P38 has been shown to have a role in cell cycle check points and DNA damage pathways. The upstream kinases, MKK3, MKK6 and SEK activate p38α by phosphorylation at Thr180 and Tyr182.
      Once activated, p38α MAP kinase has been shown to phosphorylate and activate a number of proteins including MAPKAP kinase 2 and the transcription factors ATF-2, MEF2 and MSK1.
      Product Information
      • 1. 20X Anti-pP38(T180/Y182) AlexaFluor®488 Antibody: (Part No. CS205783) 1 vial containing µL.
      • 2. 20X Anti-pATF2(T69/71) AlexaFluor®647 Antibody: (Part No.CS205784) 1 vial containing 150 µL.
      • 3. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL.
      • 4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
      • 5. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
      • 6. 1X Permeabilization Buffer: (Part No. CS203284) One bottle containing 13 mL.
      Detection methodFluorescent
      ApplicationThis FlowCellect p38 Stress Pathway Activation Detection Kit for Flow Cytometry is designed to enable a researcher a quick & easy way to detect the phosphorylation state of P38α & ATF2 by flow cytometry.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceLot to lot in-process testing on all kit components
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions4-8°C for antibodies and buffers
      Packaging Information
      Material Size25 Tests / Kit
      Transport Information
      Supplemental Information

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      Software Compatibility

      Catalogue Number Description  
      0500-4120 InCyte Software for Guava® easyCyte HT Systems Show Pricing & Availability

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      Life Science Research > Cell Analysis > guava easyCyte Flow Cytometers > Flow Cytometry Kits & Reagents