AP308F | Goat Anti-Mouse IgG Antibody, (H+L) FITC conjugate

AP308F
1 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAP308F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionGoat Anti-Mouse IgG Antibody, (H+L) FITC conjugate
      OverviewThe reagent is an affinity purified antibody from goat. The purified antibody is conjugated to fluorescein and stabilized in buffer.
      Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
      References
      Product Information
      FormatFITC
      PresentationLyophilized. Buffer = 0.02M Phosphate Buffer, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA and 0.1% Sodium azide. Reconstitute with 1 mL of sterile distilled water.
      Applications
      ApplicationDetect Mouse IgG using this Goat anti-Mouse IgG Antibody, (H+L) FITC conjugate validated for use in IF.
      Key Applications
      • Immunofluorescence
      Application NotesF/P RATIO: 4-6

      Immunohistochemistry: 1:200-1:500 (1-4)

      Immunocytochemistry: 1:200-1:500 (1-4)

      Flow Cytometry: 1 μg per 1x 10E6 cells (1,2,4) FITC absorption emission is 488-492nm, its emission peak is 520nm.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Concentration1.15 mg/mL
      HostGoat
      SpecificitySpecific for mouse IgG, heavy and light chain. The cross-reactivities of anti-mouse IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoAffinity Purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2–8°C for up to 12 months after date of receipt. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. Protect from light.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      GOAT ANTI-MOUSE IgG (H+L) FLUORESCEIN CONJUGATED SECONDARY ANTIBODY - 2377996 2377996
      GOAT ANTI-MOUSE IgG (H+L) FLUORESCEIN CONJUGATED SECONDARY ANTIBODY - 2430517 2430517
      GOAT ANTI-MOUSE IgG (H+L) FLUORESCEIN CONJUGATED SECONDARY ANTIBODY - 1997927 1997927
      GOAT ANTI-MOUSE IgG (H+L) -2664423 2664423
      GOAT ANTI-MOUSE IgG (H+L) -2730863 2730863
      GOAT ANTI-MOUSE IgG (H+L) -2763949 2763949
      GOAT ANTI-MOUSE IgG (H+L) FLUORESCEIN CONJUGATED - 2491190 2491190
      GOAT ANTI-MOUSE IgG (H+L) FLUORESCEIN CONJUGATED SECONDARY ANTIBODY 2894627
      GOAT ANTI-MOUSE IgG (H+L) FLUORESCEIN CONJUGATED SECONDARY ANTIBODY - 2189709 2189709

      References

      Reference overviewApplicationPub Med ID
      Effect of selegiline on neural stem cells differentiation: a possible role for neurotrophic factors.
      Hassanzadeh, K; Nikzaban, M; Moloudi, MR; Izadpanah, E
      Iranian journal of basic medical sciences  18  549-54  2015

      Show Abstract
      26221478 26221478
      Placentation and fetal membrane development in the South American coati, Nasua nasua (Mammalia, Carnivora, Procyonidae).
      Favaron, PO; Morini, JC; Mess, AM; Miglino, MA; Ambrósio, CE
      Reproductive biology and endocrinology : RB&E  12  57  2014

      Show Abstract
      24969476 24969476
      Transplantation of adult monkey neural stem cells into a contusion spinal cord injury model in rhesus macaque monkeys.
      Nemati, SN; Jabbari, R; Hajinasrollah, M; Zare Mehrjerdi, N; Azizi, H; Hemmesi, K; Moghiminasr, R; Azhdari, Z; Talebi, A; Mohitmafi, S; Vosough Taqi Dizaj, A; Sharifi, G; Baharvand, H; Rezaee, O; Kiani, S
      Cell journal  16  117-30  2014

      Show Abstract
      24567941 24567941
      Simultaneous downregulation of KLF5 and Fli1 is a key feature underlying systemic sclerosis.
      Noda, S; Asano, Y; Nishimura, S; Taniguchi, T; Fujiu, K; Manabe, I; Nakamura, K; Yamashita, T; Saigusa, R; Akamata, K; Takahashi, T; Ichimura, Y; Toyama, T; Tsuruta, D; Trojanowska, M; Nagai, R; Sato, S
      Nature communications  5  5797  2014

      Show Abstract
      Immunohistochemistry25504335 25504335
      Placentation in the anteaters Myrmecophaga tridactyla and Tamandua tetradactyla (Eutheria, Xenarthra).
      Mess, AM; Favaron, PO; Pfarrer, C; Osmann, C; Melo, AP; Rodrigues, RF; Ambrósio, CE; Bevilacqua, E; Miglino, MA
      Reproductive biology and endocrinology : RB&E  10  102  2012

      Show Abstract
      23199198 23199198
      Placentation in Sigmodontinae: a rodent taxon native to South America.
      Favaron, PO; Carter, AM; Ambrósio, CE; Morini, AC; Mess, AM; de Oliveira, MF; Miglino, MA
      Reproductive biology and endocrinology : RB&E  9  55  2011

      Show Abstract
      21518439 21518439
      Fcgamma receptors contribute to pyramidal cell death in the mouse hippocampus following local kainic acid injection.
      Suemitsu S, Watanabe M, Yokobayashi E, Usui S, Ishikawa T, Matsumoto Y, Yamada N, Okamoto M, Kuroda S
      Neuroscience  166  819-31 Epub 2010 Jan 13  2010

      Show Abstract
      20074624 20074624
      Comparative proteome and transcriptome analyses of embryonic stem cells during embryoid body-based differentiation.
      Ali Fathi,Mohammad Pakzad,Adele Taei,Thore C Brink,Leila Pirhaji,Guifré Ruiz,Mohammad Sharif Tabe Bordbar,Hamid Gourabi,James Adjaye,Hossein Baharvand,Ghasem Hosseini Salekdeh
      Proteomics  9  2009

      Show Abstract
      19862760 19862760
      Chorioallantoic placentation in Galea spixii (Rodentia, Caviomorpha, Caviidae).
      Oliveira, MF; Mess, A; Ambrósio, CE; Dantas, CA; Favaron, PO; Miglino, MA
      Reproductive biology and endocrinology : RB&E  6  39  2008

      Show Abstract
      18771596 18771596
      Identification of mouse embryonic stem cell-associated proteins.
      Hossein Baharvand,Ali Fathi,Hamid Gourabi,Sepideh Mollamohammadi,Ghasem Hosseini Salekdeh
      Journal of proteome research  7  2008

      Show Abstract
      18047272 18047272

      Brochure

      Title
      Western Blotting Tools

      Data Sheet

      Title
      Goat anti-Mouse IgG, (H&L) FITC Conjugated - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies