AP307F | Goat Anti-Rabbit IgG Antibody, (H+L) FITC conjugate

AP307F
1 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAP307F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionGoat Anti-Rabbit IgG Antibody, (H+L) FITC conjugate
      OverviewThe reagent is an affinity purified antibody from goat. The purified antibody is conjugated to fluorescein isothiocyanate (FITC) and stabilized in buffer
      Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
      References
      Product Information
      FormatFITC
      PresentationImmunoaffinity Purified immunoglobulin in 0.02M Phosphate Buffer, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA containing no preservatives.
      Applications
      ApplicationGoat anti-Rabbit IgG Antibody, (H+L) FITC conjugate detects level of Rabbit IgG & has been published & validated for use in IF.
      Key Applications
      • Immunofluorescence
      Application NotesImmunohistochemistry:1:200-1:500 (1-4)

      Immunocytochemistry: 1:200-1:500 (1,4)

      Flow Cytometry: 1 μg per 1x10E6 cells (1,2,4)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Concentration1.0 mg/mL
      HostGoat
      SpecificitySpecific for rabbit IgG, heavy and light chain. The cross-reactivities of anti-rabbit IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.
      Species Reactivity
      • Rabbit
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoAffinity Purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe undiluted antibody is stable at 2–8°C for 12 months. Do not store in a diluted format.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      GOAT ANTI-RABBIT IgG (H+L) FLUORESCEIN CONJUGATED SECONDARY ANTIBODY - 2379343 2379343
      GOAT ANTI-RABBIT IgG (H+L) -2664422 2664422
      GOAT ANTI-RABBIT IgG (H+L) -2697524 2697524
      GOAT ANTI-RABBIT IgG (H+L) -2705355 2705355
      GOAT ANTI-RABBIT IgG (H+L) FLUORESCEIN CONJUGATED SECONDARY ANTIBODY 2897034
      GOAT ANTI-RABBIT IgG (H+L) FLUORESCEIN CONJUGATED SECONDARY ANTIBODY - 2006360 2006360
      GOAT ANTI-RABBIT IgG (H+L) FLUORESCEIN CONJUGATED SECONDARY ANTIBODY - 2102014 2102014
      GOAT ANTI-RABBIT IgG (H+L) FLUORESCEIN CONJUGATED SECONDARY ANTIBODY - 2205924 2205924
      GOAT ANTI-RABBIT IgG (H+L) FLUORESCEIN CONJUGATED SECONDARY ANTIBODY -2484213 2484213

      References

      Reference overviewPub Med ID
      Nuclear magnetic resonance structure revealed that the human polyomavirus JC virus agnoprotein contains an α-helix encompassing the Leu/Ile/Phe-rich domain.
      Coric, P; Saribas, AS; Abou-Gharbia, M; Childers, W; White, MK; Bouaziz, S; Safak, M
      Journal of virology  88  6556-75  2014

      Show Abstract
      24672035 24672035
      Selective expression of CYP2A13 in human pancreatic α-islet cells.
      Guo, Yu, et al.
      Drug Metab. Dispos., 40: 1878-82 (2012)  2012

      Show Abstract
      22798551 22798551
      Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction.
      Hong Chang,Chen Zhang,Yi Cao
      Histochemistry and cell biology  137  2012

      Show Abstract
      22218735 22218735
      Targeting the FOXO1/KLF6 axis regulates EGFR signaling and treatment response.
      Sangodkar, J; Dhawan, NS; Melville, H; Singh, VJ; Yuan, E; Rana, H; Izadmehr, S; Farrington, C; Mazhar, S; Katz, S; Albano, T; Arnovitz, P; Okrent, R; Ohlmeyer, M; Galsky, M; Burstein, D; Zhang, D; Politi, K; Difeo, A; Narla, G
      The Journal of clinical investigation  122  2637-51  2012

      Show Abstract
      22653055 22653055
      Overexpression of caveolin-1 in lymphoblastoid TK6 cells enhances proliferation after irradiation with clinically relevant doses.
      David Barzan,Patrick Maier,W Jens Zeller,Frederik Wenz,Carsten Herskind
      Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]  186  2010

      Show Abstract
      20127227 20127227
      Identification of Vanabin-interacting protein 1 (VIP1) from blood cells of the vanadium-rich ascidian Ascidia sydneiensis samea.
      Tatsuya Ueki, Koki Shintaku, Yuki Yonekawa, Nariaki Takatsu, Hiroshi Yamada, Toshiyuki Hamada, Hiroshi Hirota, Hitoshi Michibata
      Biochimica et biophysica acta  1770  951-7  2007

      Show Abstract
      17376595 17376595
      1,25(OH)2-vitamin D3 actions on cell proliferation, size, gene expression, and receptor localization, in the HL-1 cardiac myocyte.
      Nibbelink, KA; Tishkoff, DX; Hershey, SD; Rahman, A; Simpson, RU
      The Journal of steroid biochemistry and molecular biology  103  533-7  2007

      Show Abstract
      17276054 17276054
      Androgen receptor as a licensing factor for DNA replication in androgen-sensitive prostate cancer cells.
      Litvinov, IV; Vander Griend, DJ; Antony, L; Dalrymple, S; De Marzo, AM; Drake, CG; Isaacs, JT
      Proceedings of the National Academy of Sciences of the United States of America  103  15085-90  2006

      Show Abstract
      17015840 17015840
      Hypoxic induction of vascular endothelial growth factor is selectively impaired in mice carrying the mutant SOD1 gene.
      Tetsuro Murakami, Hristelina Ilieva, Mito Shiote, Tetsuya Nagata, Isao Nagano, Mikio Shoji, Koji Abe
      Brain research  989  231-7  2003

      Show Abstract
      14556945 14556945

      Brochure

      Title
      Western Blotting Tools

      Data Sheet

      Title
      GOAT ANTI-RABBIT IgG (H+L) FLUORESCEIN CONJUGATED

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      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies