|Protein Concentration and Diafiltration by Tangential Flow Filtration -- An Overview|
|Are the TFF membranes autoclavable or steam sterilizable?||The membranes for Pellicon XL, Helicon UF, Helicon RO, spiral wound/prep/scale, Prostak UF and Pellicon 2 are not autoclavable or steam sterilizable. However, a chemical sanitization can be done; refer to the maintenance manual for these membranes.
Note: Prostak Open Channel with microporous Durapore membranes can be steam sterilized.
|What is Normalized Water Permeability?||NWP is a measure of the ability of pure water to pass through the filter membrane. NWP is a property of the TFF system and is contributed to by the membrane as well as the feed and permeate flow paths in the filter.|
|How does NWP aid in effective cleaning procedure development?||Fouling and polarization leave residues on the membrane that can reduce filter permeability. Effective cleaning removes these residues, restoring the permeability of the membrane. Ideally, NWP after cleaning should equal NWP before use although there can be variation up to +/-15% due to membrane compaction under pressure and measurement error.
NWP validates the ability of cleaning to restore filtration rate of a system.
|How is NWP measured?||NWP is measured by filtering pure water throught he system at controlled cross flow, Trans Membrane Pressure and temperature. The filtrate flow rate is measured. The flow rate is normalized to the membrane area, TMP and temperature.
Please refer to the operator's manual or contact Techical Service for further questions regarding the calculation of NWP.
|Helicon-SS50 Cartridges Installation Guide|
Custom Engineered SystemsFor chromatography systems, tangential flow filtration systems, integrated systems.
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|Biopharmaceutical Manufacturing > Downstream Processing > Ultrafiltration|
Helicon SS50 cartridges are spiral-wound ultrafiltration membrane cartridges designed for the concentration and desalting of aqueous solutions of macromolecules. Helicon SS50 cartridges offer low cost for large membrane areas and are ideally suited for tangential flow filtration (TFF) systems intended for the production of large-volume pharmaceutical products.
Each Helicon SS50 cartridge is made by wrapping alternate layers of Millipore UF membranes and plastic separator screens concentrically around a hollow core. Product enters one end of the cartridge under pressure, flowing tangentially down the cartridge axis. Ultrafiltrate (salts, water, species not rejected by the membranes) flows through the membranes into permeate channels and spirals to the central core, from which it is removed. Retentate flows out the opposite end of the cartridge.
- Concentration and desalting of:
– Other biopolymer solutions
- Concentration and desalting of, and alcohol removal from:
– Plasma fractions
- Depyrogenation of solutions of low molecular weight compounds:
|Materials of Construction|
|PL Membrane||Regenerated Cellulose (RC)|
|PT Membrane||Regenerated Cellulose (RC)|
|Screens||Feed/retentate: polyethylene (PE)
Permeate: polypropylene (PP)
|Center Tube, Shell and Disk; Fold Reinforcement||Center Tube, Shell and Chevron Support Disk: polysulfone
Fold Reinforcement: Polyethylene
|Chevron Seal and O-rings||EPDM|
|Pressure, bar (psi)|
|Normal Operating Range||0–7 (0–100)|
|Maximum Inlet if permitted by housing||0–6.9 bar (0–100 psi)|
|pH Range - Intermittent (up to 250 h)|
|pH Range - continuous|
|PT Series||pH2 – pH12|
|PL Series||pH2 – pH12|