14-155 | Histone H1 Protein, 20 mg

14-155
20 mg  
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      Overview

      Replacement Information

      Special Offers

      Key Spec Table

      Key ApplicationsEntrez Gene NumberUni Prot Number
      KANM_005321.2P10412
      Description
      Catalogue Number14-155
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHistone H1 Protein, 20 mg
      Alternate Names
      • Linker Histone H1
      Background InformationH1 and related linker histones are important both for maintenance of higher-order chromatin structure and for the regulation of gene expression. Nucleosomal stabilization is provided through the binding of histone H1 to the nucleosomal dyad and the linker DNA entering and exiting the core particle. Additional experimental evidence illustrates the influence of histone H1 on chromatin arrangement and compaction. The family of histone H1 proteins contains five somatic variants (H1.1, H1.2, H1.3, H1.4 and H1.5) which are expressed in nearly all cell types.
      References
      Product Information
      Applications
      ApplicationPurified bovine Histone H1 protein for use as a substrate in histone modification assays (HAT, HDAC, DNMT) and chromatin assembly studies.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceLysine rich fraction from calf thymus
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H1E
      • dJ221C16.5
      • H1.4
      • H1F4
      • MGC116819
      Purification MethodSterilized through a 0.2 micron membrane filter
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10412 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
      SIZE: 219 amino acids; 21865 Da
      SUBCELLULAR LOCATION: Nucleus.
      SIMILARITY: SwissProt: P10412 ## Belongs to the histone H1/H5 family.
      MISCELLANEOUS: This variant accounts for 60% of histone H1.
      Molecular Weight32kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Kinase assay using cdk2/cyclin A, active (14-448) to phosphorylate Histone H1.
      Sales RestrictionsThis product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size20 mg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Histone H1 2475705
      Histone H1 (substrate for kinase assays) - DAM1467560 DAM1467560
      Histone H1 - 2127187 2127187
      Histone H1 - 2460946 2460946
      Histone H1 - 16294 16294
      Histone H1 - 18939 18939
      Histone H1 - 19847 19847
      Histone H1 - 2019544 2019544
      Histone H1 - 21706 21706
      Histone H1 - 2194170 2194170

      References

      Reference overviewApplicationPub Med ID
      Replication of minute virus of mice in murine cells is facilitated by virally induced depletion of p21.
      Adeyemi, RO; Pintel, DJ
      Journal of virology  86  8328-32  2012

      Show Abstract
      22623787 22623787
      Protein kinase A-mediated serine 35 phosphorylation dissociates histone H1.4 from mitotic chromosome.
      Chi-Shuen Chu,Pang-Hung Hsu,Pei-Wen Lo,Elisabeth Scheer,Laszlo Tora,Hang-Jen Tsai,Ming-Daw Tsai,Li-Jung Juan
      The Journal of biological chemistry  286  2011

      Show Abstract Full Text Article
      21852232 21852232
      CR6-interacting factor 1 interacts with Gadd45 family proteins and modulates the cell cycle
      Chung, H. K., et al
      J Biol Chem, 278:28079-88 (2003)  2003

      Kinase Assay12716909 12716909
      Rho-dependent Rho kinase activation increases CD44 surface expression and bone resorption in osteoclasts.
      Chellaiah, MA; Biswas, RS; Rittling, SR; Denhardt, DT; Hruska, KA
      The Journal of biological chemistry  278  29086-97  2003

      Show Abstract
      Kinase Assay12730217 12730217
      Pim3 negatively regulates glucose-stimulated insulin secretion.
      Gregory Vlacich,Martijn C Nawijn,Gene C Webb,Donald F Steiner
      Islets  2  2001

      Show Abstract Full Text Article
      21099329 21099329
      Human Prk is a conserved protein serine/threonine kinase involved in regulating M phase functions
      Ouyang, B., et al
      J Biol Chem, 272:28646-51 (1997)  1997

      Kinase Assay9353331 9353331