EZHAP0A4-73K | Human Apo AIV ELISA

96-well plate  
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      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Apo AIVHRIAColorimetric, Radioactive
      Catalogue NumberEZHAP0A4-73K
      DescriptionHuman Apo AIV ELISA
      Background InformationApolipoprotein AIV (Apo AIV) is a glycoprotein synthesized by the human intestine. Synthesis appears to be stimulated by the absorption of high density lipoproteins that are rich in triglycerides rather than by the uptake or re-esterification of fatty acids. The formation of chylomicrons acts as a signal for the induction of Apo AIV synthesis a signal enhanced by a factor from the ileum, probably PYY. Apo AIV alters the activity of the key enzymes (LPL and LCAT) of lipoprotein metabolism and cholesterol efflux from extrahepatic tissues. Also, since stimulation of intestinal synthesis and secretion by lipid absorption are rapid, Apo AIV likely plays a role in the short-term regulation of food intake. Other evidence suggests involvement in the long-term regulation of both food intake and body weight. Chronic ingestion of high-fat diet blunts the intestinal Apo AIV response to lipid feeding and, consequently, may explain why the chronic ingestion of a high-fat diet predisposes both animals and humans to obesity.
      Product Information
      Detection methodColorimetric Radioactive
      Precision, %
      • Inter-assay 11 – 13%
      • Intra-assay 4 – 6%
      ApplicationThis Human Apo AIV ELISA is used to measure & quantify Apo AIV levels in Metabolism research.
      Key Applications
      • Radioimmunoassay
      Application NotesThis is a 4-hour assay at room temperature requiring 20 μL human serum, plasma, and tissue culture samples.
      Biological Information
      SpecificityThe antibodies used in this kit show a high cross-reactivity to non-human primate samples. However, no species cross-reactivity is obseved in the assay to rat, mouse, canine, porcine, or sheep samples.

      No cross reactivity was observed to the following human analytes:
      Apo A1, Apo AII, Apo AV, Apo B, Apo CII, Apo CIII, Apo E, cytokines, adipokines or endocrine hormones.
      Species Reactivity
      • Human
      Analytes Available
      • Apo AIV
      • 91-103%
      Physicochemical Information
      • 0.08 μg/mL
      Linearity of Dilution77-100%
      Standard Curve Range
      • 0.8 - 5.0 μg/mL
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well plate
      Transport Information
      Supplemental Information