EZHI-14K | Human Insulin ELISA

EZHI-14K
96-well strip plate  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      InsulinHELISAFluorescent, Colorimetric
      Description
      Catalogue NumberEZHI-14K
      DescriptionHuman Insulin ELISA
      Background InformationInsulin, a 5.8 kDa hormone, is secreted by the islet beta cells in the pancreas.
      References
      Product Information
      Detection methodFluorescent Colorimetric
      Precision, %
      • Inter-assay: 9.1–11.4
      • Intra-assay: 4.6–7.0
      Applications
      ApplicationThis Human Insulin ELISA is used to measure & quantify Insulin levels in Metabolism & Endocrine research.
      Key Applications
      • ELISA
      Application Notes2 hour assay, room temperature Sample ≤ 20 μL serum, plasma, or tissue culture medium
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • Insulin
      Entrez Gene Number
      Entrez Gene SummaryAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
      Gene Symbol
      • INS
      • proinsulin
      • insulin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
      SIZE: 110 amino acids; 11981 Da
      SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
      SUBCELLULAR LOCATION: Secreted.
      DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
      SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
      Accuracy
      • 76–103%
      Physicochemical Information
      Linearity of Dilution96–112%
      Standard Curve Range
      • 2–200 μU/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      HUMAN INSULIN ELISA KIT - 96-Well Plate

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Long-term, progressive, aerobic training increases adiponectin in middle-aged, overweight, untrained males and females.
      Mujumdar PP, Duerksen-Hughes PJ, Firek AF, Hessinger DA
      Scand J Clin Lab Invest  71  101-7. Epub 2011 Jan 27.  2011

      Show Abstract
      21271804 21271804
      Non-invasive Measurement of Plasma Glucose from Exhaled Breath in Healthy and Type 1 Diabetic Mellitus Subjects.
      Minh TD, Oliver SR, Ngo J, Flores RL, Midyett J, Meinardi S, Carlson MK, Rowland FS, Blake DR, Galassetti PR
      Am J Physiol Endocrinol Metab  2011

      Show Abstract
      21467303 21467303
      Role of baseline leptin and ghrelin levels on body weight and fat mass Changes after an energy-restricted diet intervention in
      Labayen I, Ortega FB, Ruiz JR, Lasa A, Simón E, Margareto J
      The Journal of clinical endocrinology and metabolism  96  E996-1000. Epub 2011 Apr 6.  2011

      21470990 21470990
      The effects of aerobic, resistance, and combined exercise on metabolic control, inflammatory markers, adipocytokines, and muscle insulin signaling in patients with type 2 diabetes mellitus.
      Jorge ML, de Oliveira VN, Resende NM, Paraiso LF, Calixto A, Diniz AL, Resende ES, Ropelle ER, Carvalheira JB, Espindola FS, Jorge PT, Geloneze B
      Metabolism  2011

      Show Abstract
      21377179 21377179
      Hypobaric hypoxia causes body weight reduction in obese subjects.
      Florian J Lippl,Sonja Neubauer,Susanne Schipfer,Nicole Lichter,Amanda Tufman,Bärbel Otto,Rainald Fischer
      Obesity (Silver Spring, Md.)  18  2010

      Show Abstract
      20134417 20134417
      Dose-dependent relationship between severity of pediatric obesity and blunting of the growth hormone response to exercise.
      Stacy R Oliver,Jaime S Rosa,Timothy D C Minh,Andria M Pontello,Rebecca L Flores,Marcia Barnett,Pietro R Galassetti
      Journal of applied physiology (Bethesda, Md. : 1985)  108  2010

      Show Abstract Full Text Article
      19875716 19875716
      Plasma levels of MCP-1 and adiponectin in obstructive sleep apnea syndrome.
      Kim J, Lee CH, Park CS, Kim BG, Kim SW, Cho JH
      Arch Otolaryngol Head Neck Surg  136  896-9.  2010

      Show Abstract
      20855682 20855682
      Total and high molecular weight adiponectin in patients with coronary artery disease.
      Ayman El-Menyar, Nasser Rizk, Abdulrahman D Al Nabti, Sana Abu Hassira, Rajvir Singh, Mohamed Osman Abdel Rahman, Jassim Al Suwaidi
      Journal of cardiovascular medicine (Hagerstown, Md.)  10  310-5  2009

      Show Abstract
      19430341 19430341
      Small interfering RNA-mediated suppression of proislet amyloid polypeptide expression inhibits islet amyloid formation and enhances survival of human islets in culture.
      Lucy Marzban, Alejandra Tomas, Thomas C Becker, Lawrence Rosenberg, Jose Oberholzer, Paul E Fraser, Philippe A Halban, C Bruce Verchere
      Diabetes  57  3045-55  2008

      Show Abstract Full Text Article
      18694973 18694973
      Acute d-psicose administration decreases the glycemic responses to an oral maltodextrin tolerance test in normal adults.
      Tetsuo Iida, Yuka Kishimoto, Yuko Yoshikawa, Noriko Hayashi, Kazuhiro Okuma, Mikiko Tohi, Kanako Yagi, Tatsuhiro Matsuo, Ken Izumori
      Journal of nutritional science and vitaminology  54  511-4  2008

      Show Abstract
      19155592 19155592

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits