EZHL-80BK | Human Leptin "Dual Range" ELISA- Space Saver (Bulk) Packaging

EZHL-80BK
96-well strip plate  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      LeptinHELISAFluorescent, Colorimetric
      Description
      Catalogue NumberEZHL-80BK
      DescriptionHuman Leptin "Dual Range" ELISA- Space Saver (Bulk) Packaging
      Background InformationLeptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
      References
      Product Information
      Detection methodFluorescent Colorimetric
      Precision, %
      • Inter-assay: 2.6–6.2 Sensitive Assay 1.3–8.6
      • Intra-assay: 2.6–4.6 Sensitive Assay 1.4–4.9
      Applications
      ApplicationThis Human Leptin "Dual Range" ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • ELISA
      Application NotesRoom temperature, 3.5 hour assay 25 μL sample size (Sensitive Assay: 50μL)
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • Leptin
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.
      Gene Symbol
      • LEP
      • leptin
      • OB
      • obesity
      • OBS
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P41159 # May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
      SIZE: 167 amino acids; 18641 Da
      SUBUNIT: Interacts with SIGLEC6.
      SUBCELLULAR LOCATION: Secreted.
      DISEASE:SwissProt: P41159 # Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].
      SIMILARITY: SwissProt: P41159 ## Belongs to the leptin family.
      Accuracy
      • 94–114%
      • Sensitive Assay 86–102%
      Physicochemical Information
      Sensitivity
      • 0.5 ng/mL
      • Sensitive Assay 0.125 ng/mL
      Linearity of Dilution80–105% Sensitive Assay 96–125%
      Standard Curve Range
      • 0.5–100 ng/mL
      • Sensitive Assay 0.125–20 ng/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits