|Pellicon® Laboratory Systems, Holders and Assemblies|
|Pellicon® XL 50 Cassette and Labscale TFF System|
|Can I use TFF systems to separate two proteins based on a significant difference in their respective molecular weights?||As a general rule ultrafiltration does not provide high efficiency separations of protein mixtures. However, it may be possible to effect this type of separation between two proteins that have at least 10 X difference in molecular weight and if there is an ultrafiltration membrane available with a NMWL rating between the molecular weight of the two proteins. These separations are also generally more successful with dilute protein solutions and if run at low pressure.|
|How many times can I reuse TFF membranes?||It is possible to reuse both ultrafiltration and microporous membranes used in Millipore Tangential flow systems (Miniplate, Prep Scale etc.). The number of uses for any given set of membranes is directly related to the effectiveness of the cleaning and storage protocols used. Please refer to the TFF Maintenance Guides for suggested cleaning and storage procedures.|
|How do I dampen the pulse on a Labscale system?||The best method is to apply a restriction (pressure drop) in the pump outlet tube. Use a small pinch valve (one supplied with the PXL device) and pinch the pump outlet tube to dampen the pulsation. The closer you put the clamp to the feed inlet of the manifold, the more effective the dampening will be. The pinch valve effectively reduces the feed flow pulsation caused by the reciprocating diaphragm pump. The pinch valve is applied to the pump outlet tube and is therefore upstream of the feed pressure gauge and PXL device. Flow and feed pressure to the device is not effected once the pump speed and pinch valve have been set.
Procedure: Set pressure drop across PXL device
Adjust pinch valve to minimize feed gauge pulsation
Pump speed may or may not need to be adjusted to maintain feed pressure
By creating a pressure drop prior to the Labscale Reservoir manifold, you create a driving force to maintain flow even while the pump is in the suction mode. A pressure drop of approximately 10 psi across the pinch valve works quite well. The pinch valve is required to adjust the restriction depending on the flow rate
|Can the Labscale TFF System accommodate more than one Pellicon XL?||PRODUCT:
Multimanifold Kit for Labscale TFF System
The Labscale Multimanifold Kit is designed to accommodate up to 3 Pellicon XL devices and mount directly onto the Labscale Reservoir. It may also be used with a peristaltic pump for those customers without a Labscale Reservoir.
It is intended to be used for either membrane selection by choosing devices with different membranes or to increase surface area by choosing devices with the same membrane.
The Labscale Multi-Manifold Kit includes 2 multi-port manifolds with assorted tubing fittings. Manifold fittings include male and female luer lock. Assorted fittings included male and female luer plugs, male to female luer lock elbows, and male luer lock to 1/8" barb.
Materials of Construction
|Labscale Multi Manifold Kit Installation Instructions|
|Labscale™ Tangential Flow Filtration System|