FCYTOMAG-20K | MILLIPLEX MAP Feline Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

FCYTOMAG-20K

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Overview

Replacement Information

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Please see FCYTMAG-20K-PMX as an alternative.

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
sFas, Flt-3 ligand, GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-12 (p40), IL-13, IL-18, KC, MCP-1, PDGF-BB, RANTES, SCF, SDF-1, TNF-αFeMplexLuminex xMAP
Description
Catalogue NumberFCYTOMAG-20K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Feline Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
Background InformationCytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-Assay: <20
  • Intra-Assay: <15
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in feline serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation assay (2 hours is not recommended) with a 1-hour incubation with detection antibodies requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the Feline Cytokine/Chemokine panel analytes in serum, plasma or tissue/cell lysate and culture supernatant.
Biological Information
Species Reactivity
  • Feline
Analytes Available
  • sFas
  • Flt-3 ligand
  • GM-CSF
  • IFN-γ
  • IL-1β
  • IL-2
  • IL-4
  • IL-6
  • IL-8
  • IL-12 (p40)
  • IL-13
  • IL-18
  • KC
  • MCP-1
  • PDGF-BB
  • RANTES
  • SCF
  • SDF-1
  • TNF-α
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • 96 - 114%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivites for individual cytokines/chemokines
Standard Curve Range
  • sFas, IL-2, GM-CSF, IL-8, IL-12 (p40), IL-13, TNFα: 13.7 – 10,000 pg/mL
  • Flt3 Ligand: 6.9 – 5,000 pg/mL
  • IFNγ, IL-4, IL-6, SCF, SDF-1: 68.6 – 50,000 pg/mL
  • IL-1β: 34.3 – 25,000 pg/mL
  • IL-18, MCP-1: 137.2 – 100,000 pg/mL
  • KC, RANTES: 2.7 – 2,000 pg/mL
  • PDGF-BB: 274.3 – 200,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
Custom Immunoassay Menu of Services
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
FCYTOMAG-20K xPonent Protocol Template Version Magpix
FCYTOMAG-20K xPonent Standard Template Version Magpix
Quality Control Ranges - Milliplex Feline Cytokin/Chemokine Magnetic Bead Panel

Data Sheet

Title
Full Service Custom-built Assays

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain
MILLIPLEX® MAP Cell Signaling Wall Chart (MM)
Multiplex Assays of Fifteen Human Complement Factors