HNCSMAG-35K | MILLIPLEX MAP Human Circadian/Stress Magnetic Bead Panel - Multiplex Assay

HNCSMAG-35K
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Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Cortisol, MelatoninHMplexLuminex xMAP
Description
Catalogue NumberHNCSMAG-35K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Circadian/Stress Magnetic Bead Panel - Multiplex Assay
OverviewThe MILLIPLEX® MAP Human Circadian/Stress Magnetic Bead Panel is to be used for the simultaneous quantification of Cortisol and Melatonin. This is a configurable assay, and either or both analytes can be selected. This kit may be used for the analysis of human serum, plasma, tissue/cell lysate, culture supernatant samples, or cerebrospinal fluid (CSF).

Serum or plasma samples should be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with this kit.
Background InformationThe MILLIPLEX® MAP Human Circadian/Stress Magnetic Bead Panel enables you to focus on the relevance and therapeutic potential of Melatonin and Cortisol in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.
References
Product Information
Components
  • Human Circadian/Stress Standard, 1 vial, lyophilized
  • Human Circadian/Stress Quality Control 1, 1 vial, lyophilized
  • Human Circadian/Stress Quality Control 2, 1 vial, lyophilized
  • Assay Buffer, 2 bottles, 30 mL ea
  • Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
  • Human Circadian/Stress Primary Antibodies, 1 bottle, 3.5 mL
  • Human Circadian/Stress Detection Antibodies, 1 bottle, 5.5 mL
  • Human Circadian/Stress Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea
  • Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
  • Mixing Bottle, 1 bottle
  • Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 25% CV
  • Intra-assay: < 15% CV
Panel TypeMAGNETIC Neuroscience
Applications
ApplicationThe MILLIPLEX® MAP Human Circadian/Stress Panel is a 2-plex kit containing all reagents needed for simultaneous quantification of Cortisol and/or Melatonin in serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples on Luminex® systems.
Key Applications
  • Multiplexing
Application NotesEMD Millipore’s MILLIPLEX® MAP Human Circadian/Stress Magnetic Bead Panel is a 2-plex kit to be used for the simultaneous quantification of cortisol and/or melatonin in serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples.
• This is a competitive assay. An overnight (16-18 hours) incubation at 2-8°C is recommended.
• This assay requires 50 μL extracted serum or plasma sample per well or 50 μL neat CSF or culture supernatant per well.
• Sample extraction protocols are required for serum/plasma samples. Other sample types (e.g. CSF, culture supernatant, saliva, etc.) do not require sample extraction.
• A 1:4 sample dilution is recommended for extracted serum/plasma samples.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Human
Analytes Available
  • Cortisol
  • Melatonin
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • Spike Recovery for Cortisol and Melatonin is 120% and 129%, repsectively.
Physicochemical Information
Sensitivity
  • Cortisol: 686 pg/mL (MinDC+2SD)
  • Melatonin: 4.3 pg/mL (MinDC+2SD)
Standard Curve Range
  • Cortisol: 686 – 500,000 pg/mL
  • Melatonin: 3.4 – 2,500 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human Circadian/Stress Magnetic Bead Panel

SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
Immunoassay and Multiplex Kits: Analyte Quarterly Vol. 2, 2016
Protein Detection Platform and Services Brochure

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Ranges: MILLIPLEX® MAP Human Circadian/Stress Magnetic Bead Panel

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain
Multiplex Assays of Fifteen Human Complement Factors