ECM492 | MMP-2 Immunoassay Kit, pro-form only

ECM492
100 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey Applications
      MMP-2HELISA
      Description
      Catalogue NumberECM492
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionMMP-2 Immunoassay Kit, pro-form only
      OverviewMatrix metalloproteinase 2 (72 kDa gelatinase, gelatinase A, MMP-2) has been identified in a number of tissues, cells and blood. The precursor form of MMP-2 (pro-MMP-2) is tightly associated with tissue inhibitor of metalloproteinases-2 (TIMP-2) and regulated autoactivation.

      Pro-MMP-2 may be quantitated using this sensitive immunoassay against

      MMP-2

      Test Principle:

      The MMP-2 immunoassay kit utilizes antibodies immobilized on a bead matrix, in combination with enzyme-labeled antibodies, directed against different antigenic sites on the same MMP-2 molecule. Upon addition of an MMP-2-containing specimen, the result is an MMP-2 molecule being sandwiched between the solid phase and enzyme labeled antibodies. After removing unbound enzyme-labeled antibody, the bead containing the sandwich is incubated with enzyme substrate and o-phenylenediamine, resulting in the development of color. The activity of peroxidase enzyme is proportional to the amount of antigen, MMP-2, so that MMP-2 concentration in specimens can be determined from a standard curve.

      Application:

      The MMP-2 immunoassay kit is useful for the determination of MMP-2 (pro-MMP-2) levels in fresh human sera.

      The values measured by using this kit represent pro-MMP-2 concentrations, because pro-MMP-2 is used as a standard in this kit. This system recognizes both free pro-MMP-2 and pro-MMP-2 complexed with TIMP-2. It does not recognize active MMP-2. Contents of this kit are sufficient for assay of 100 samples, including standard curve. Testing of samples in duplicate or triplicate is strongly recommended. This kit is intended for research use only; not for diagnostic or therapeutic applications.
      Materials Required but Not Delivered· Pipette (10 mL)

      · Pipettors & tips capable of accurately measuring 50-1000 μL

      · Dispensor (3 mL)

      · Test tubes (internal diameter 10-13 mm; length 60-75 mm)

      · Aspirator

      · Graduated cylinder (50 mL, 1000 mL)

      · Spectrophotometer (492 nm)
      References
      Product Information
      Components
      • Anti-MMP-2 Coated Beads - One (1) bottle containing 100 anti-MMP-2 coated polystyrene beads.
      • Concentrated Enzyme Labeled Antibody Solution - One (1) 1.2 mL bottle containing MMP-2 antibody.
      • Color Reagent (Lyophilized) -Four (4) bottles o-Phenylenediamine (OPD), each sufficient to make one 12 mL bottle of color reagent.
      • Substrate Solution - One (1) 50 mL bottle 0.02% Hydrogen Peroxide.
      • Stop Solution - Two (2) bottles, sufficient to make 100 mL 1.3 N Sulfuric acid per bottle.
      • Buffer Reagent (Lyophilized) - One (1) 50 mL bottle BSA in sodium phosphate buffer.
      • MMP-2 Standard - Two (2) bottles, sufficient to make 0.5 mL Human pro-MMP-2 per bottle.
      • Concentrated Washing Solution - Two bottles, sufficient to make 1L Sodium phosphate buffer, pH 7.0, per bottle.
      Applications
      ApplicationThe MMP-2 immunoassay kit utilizes antibodies immobilized on a bead matrix, in combination with enzyme-labeled antibodies, directed against different antigenic sites on the same MMP-2 molecule.
      Key Applications
      • ELISA
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • MMP-2
      Entrez Gene Number
      Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome.
      Gene Symbol
      • MMP2
      • TBE-1
      • MMP-II
      • MONA
      • MMP-2
      • CLG4A
      • CLG4
      • EC 3.4.24.24
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08253 # In addition to gelatin and collagens, it cleaves KiSS1 at a Gly- -Leu bond.
      COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
      SIZE: 660 amino acids; 73882 Da
      SUBUNIT: Ligand for integrin alpha-V/beta-3.
      TISSUE SPECIFICITY: Produced by normal skin fibroblasts.
      DOMAIN: SwissProt: P08253 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
      PTM: The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT- MMP3).
      DISEASE: SwissProt: P08253 # Defects in MMP2 are the cause of multicentric osteolysis nodulosis and arthropathy (MONA) [MIM:605156]. Inherited osteolyses or 'vanishing bone' syndromes are rare disorders of unknown etiology characterized by destruction and resorption of affected bones. MONA is an autosomal recessive osteolysis with multicentric involvement characterized by carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies. & Defects in MMP2 are the cause of Winchester syndrome [MIM:277950]. Winchester syndrome is an autosomal recessive osteolysis syndrome. Winchester syndrome is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. The clinical and molecular findings suggest that Winchester syndrome and MONA are allelic disorders that form a continuous clinical spectrum.
      SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Japan.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore kit at 2-8°C.

      Precautions:

      · All specimens should be handled as potentially infectious.

      · Do not freeze reagents

      · Do not combine reagents from different lots.

      · Stop solution (1.3 N Sulfuric acid) should be handled with caution.

      · Do not allow Coloring or Stop Solution to come into contact with metal.

      · Do not use sera which were frequently frozen and thawed.

      · All reagents should be brought to ambient temperature before use. Gently stir each bottle of liquid reagent. Do not shake reagent bottles.

      · Use pipette with disposable tips for pipetting standards and specimens.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overviewPub Med ID
      Prognostic value of matrix metalloproteinases (MMP-2 and MMP-9) in Hodgkin's and non-Hodgkin's lymphoma.
      B Hazar,G Polat,E Seyrek,O Ba?dato?l?lu,A Kanik,N Tiftik
      International journal of clinical practice  58  2004

      Show Abstract
      15055861 15055861

      Brochure

      Title
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      Data Sheet

      Title
      MMP-2 Immunoassay Kit, pro-form only - Data Sheet

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      User Guides

      Title
      MMP-2 Immunoassay Kit