FCMAB180F | Milli-Mark™ Anti-CD13-FITC Antibody, clone WM-47

FCMAB180F
100 tests  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMFITCMonoclonal Antibody
      Description
      Catalogue NumberFCMAB180F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-CD13-FITC Antibody, clone WM-47
      Alternate Names
      • Aminopeptidase N (AP-N), hAPN, Alanyl aminopeptidase, Microsomal aminopeptidase, Aminopeptidase M (AP-M), gp150, Myeloid plasma membrane glycoprotein CD13
      Background InformationCD13 is a 150 kDa Type II integral membrane protein composed of 967 amino acids (Turni, 2002; Ashmun, 1995; Look, 1989). CD13 is identical in structure to aminopeptidase N, a membrane-bound zinc-binding metalloprotease (Turni, 2002). CD13 is expressed on the surface of committed granulocyte-monocyte progenitors (CFU-GM) and by cells of the granulocyte and monocyte lineages at all stages of differentiation (Turni, 2002; Ashmun, 1995; Look, 1989). The antigen is also expressed on a small proportion of large granular lymphocytes, but not on B or T cells (Ashmun, 1995; Look, 1989). Further, CD13 is expressed on some non-hematopoietic cells including fibroblasts, endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining the bile duct canaliculi (Turni, 2002; Ashmun, 1995; Look, 1989).
      Anti-CD13, WM-47, was included in the Fourth and Fifth International Workshop and Conference on Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD 13 (Ashmun, 1995; Look, 1989).
      References
      Product Information
      FormatFITC
      Control
      • PBMCs
      PresentationPurified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
      Applications
      ApplicationDetect CD13 using this Milli-Mark Anti-CD13-FITC Antibody, clone WM-47 validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenLeukaemic cells from a case of chronic myeloid leukaemia (CML).
      CloneWM-47
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityAntibody recognizes Human CD13 antigen.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight 150 kDa (Turni, 2002; Ashmun, 1995; Look, 1989)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using human PBMCs
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Milli-Mark Anti-CD13-FITC, clone WM-47 - 2140070 2140070
      Milli-Mark Anti-CD13-FITC, clone WM-47 - 2092946 2092946
      Milli-Mark Anti-CD13-FITC, clone WM-47 - 2199656 2199656
      Milli-Mark Anti-CD13-FITC, clone WM-47 - NG1940534 NG1940534
      Milli-Mark Anti-CD13-FITC, clone WM-47 - NRG1761329 NRG1761329
      Milli-Mark™ Anti-CD13-FITC, clone WM-47 3076512
      Milli-Mark™ Anti-CD13-FITC, -2586534 2586534
      Milli-Mark™ Anti-CD13-FITC, -2591349 2591349
      Milli-Mark™ Anti-CD13-FITC, -2603848 2603848
      Milli-Mark™ Anti-CD13-FITC, -2643582 2643582

      Brochure

      Title
      Innovative Solutions for Flow Cytometry Analysis