FCMAB218F | Milli-Mark® Anti-CD19-FITC Antibody, clone 6D5

100 tests  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFCRFITCMonoclonal Antibody
      Catalogue NumberFCMAB218F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark® Anti-CD19-FITC Antibody, clone 6D5
      Alternate Names
      • B-lymphocyte antigen CD19
      • Differentiation antigen CD19
      • B4, Leu-12
      Background InformationCD19 is a member of the immunoglobulin superfamily which is expressed throughout B lymphocyte development, from early pro-B cell stages and into the mature B-cell stages during development. Also known as B4, CD19+ expression is found on mouse splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. CD19 plays an integral role in B lymphocyte development, B-cell activation, and in the maturation of memory B cells. Monoclonal antibody 6D5 recognizes the same epitope as the published clone, 1D3.
      Product Information
      • mouse splenocytes
      PresentationPurified rat monoclonal IgG2ak conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      ApplicationDetect CD19 using this Milli-Mark Anti-CD19-FITC Antibody, clone 6D5 validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenFull-length murine CD19 cDNA cloned into a vector and expressed in K562 human erythroleukemia cells.
      SpecificityAntibody recognizes mouse CD19.
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cd19
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

      SUBUNIT STRUCTURE: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-346 and/or Tyr-376. Interacts with PLCG2 when phosphorylated on Tyr-402 By similarity.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      POST-TRANSLATIONAL MODIFICATION: Phosphorylated upon DNA damage, probably by ATM or ATR.

      SEQUENCE SIMILARITIES: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Molecular Weight60 kDa Calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using mouse splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies