FCMAB176F | Milli-Mark Anti-CD8 -FITC Antibody, clone DK25

FCMAB176F
100 tests  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMFITCMonoclonal Antibody
      Description
      Catalogue NumberFCMAB176F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-CD8 -FITC Antibody, clone DK25
      Background InformationCD8 is a 68 kDa disulphide-linked, transmembrane glycoprotein. The CD8 molecule serves as a receptor for MHC class I molecules and can mediate a function as a coreceptor in TCR-ligand binding and T-cell activation. Its cytoplasmic part is associated with the p56Ick tyrosine kinase (Nakuchi, 1997). CD8 is expressed by the great majority of cortical thymocytes and approximately 30% of medullary thymocytes, and by class I major histocompatibility complex restricted, mature suppressor/cytotoxic T cells. In addition a proportion of gamma delta T cells and NK cells express CD8 (Nakuchi, 1997). Thus, CD8 is expressed by about 25-35% of peripheral T cells, and about 30% of NK cells express low levels of CD8 (Leong, 1999). The specificity of Anti-CD8, DK25, is equivalent to that of the CD8-clustered antibodies OKT8 and Leu-2a (Stein, 1984).
      References
      Product Information
      FormatFITC
      Control
      • Human Lympnocytes.
      PresentationPurified Mouse monoclonal IgG1k conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2
      Applications
      ApplicationMilli-Mark Anti-CD8 -FITC Antibody, clone DK25 detects level of CD8 & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      CloneDK25
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityAntibody recognizes Human CD8 antigen.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight 68 kDa (Nakuchi, 1997)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytomatry using human lymphocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Milli-Mark Anti-CD8 -FITC, clone DK25 - 2151861 2151861
      Milli-Mark Anti-CD8 -FITC, clone DK25 - 1983985 1983985
      Milli-Mark Anti-CD8 -FITC, clone DK25 - 2204950 2204950
      Milli-Mark Anti-CD8 -FITC, clone DK25 - NRG1761315 NRG1761315
      Milli-Mark™ Anti-CD8 -FITC, -2604222 2604222
      Milli-Mark™ Anti-CD8 -FITC, -2632076 2632076
      Milli-Mark™ Anti-CD8 -FITC, -2753983 2753983
      Milli-Mark™ Anti-CD8 -FITC, -2808208 2808208
      Milli-Mark™ Anti-CD8 -FITC, clone DK25 2476798
      Milli-Mark™ Anti-CD8 -FITC, clone DK25 - 2561276 2561276

      Brochure

      Title
      Innovative Solutions for Flow Cytometry Analysis

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies