FCMAB176F Milli-Mark Anti-CD8 -FITC Antibody, clone DK25

100 tests  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMFITCMonoclonal Antibody
      Catalogue NumberFCMAB176F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-CD8 -FITC Antibody, clone DK25
      Background InformationCD8 is a 68 kDa disulphide-linked, transmembrane glycoprotein. The CD8 molecule serves as a receptor for MHC class I molecules and can mediate a function as a coreceptor in TCR-ligand binding and T-cell activation. Its cytoplasmic part is associated with the p56Ick tyrosine kinase (Nakuchi, 1997). CD8 is expressed by the great majority of cortical thymocytes and approximately 30% of medullary thymocytes, and by class I major histocompatibility complex restricted, mature suppressor/cytotoxic T cells. In addition a proportion of gamma delta T cells and NK cells express CD8 (Nakuchi, 1997). Thus, CD8 is expressed by about 25-35% of peripheral T cells, and about 30% of NK cells express low levels of CD8 (Leong, 1999). The specificity of Anti-CD8, DK25, is equivalent to that of the CD8-clustered antibodies OKT8 and Leu-2a (Stein, 1984).
      Product Information
      • Human Lympnocytes.
      PresentationPurified Mouse monoclonal IgG1k conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2
      ApplicationMilli-Mark Anti-CD8 -FITC Antibody, clone DK25 detects level of CD8 & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityAntibody recognizes Human CD8 antigen.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight 68 kDa (Nakuchi, 1997)
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytomatry using human lymphocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Milli-Mark Anti-CD8 -FITC, clone DK25 - 21518612151861
      Milli-Mark Anti-CD8 -FITC, clone DK25 - 19839851983985
      Milli-Mark Anti-CD8 -FITC, clone DK25 - 22049502204950
      Milli-Mark Anti-CD8 -FITC, clone DK25 - NRG1761315NRG1761315
      Milli-Mark™ Anti-CD8 -FITC, clone DK253069706
      Milli-Mark™ Anti-CD8 -FITC, -26042222604222
      Milli-Mark™ Anti-CD8 -FITC, -26320762632076
      Milli-Mark™ Anti-CD8 -FITC, -27539832753983
      Milli-Mark™ Anti-CD8 -FITC, -28082082808208
      Milli-Mark™ Anti-CD8 -FITC, clone DK252476798


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      Life Science Research > Antibodies and Assays > Primary Antibodies