FCMAB102P | Milli-Mark Anti-Cyclin B1-PE Antibody, clone GNS3 (8A5D12)

FCMAB102P
100 tests  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, FCMPhycoerythrinMonoclonal Antibody
      Description
      Catalogue NumberFCMAB102P
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-Cyclin B1-PE Antibody, clone GNS3 (8A5D12)
      Alternate Names
      • G2/mitotic-specific cyclin B1
        cyclin B1
      Background InformationRegulation of cell cycle progression in eukaryotic cells depends on the expression of cyclin proteins. These proteins are the regulatory subunits of the cell cycle-dependent kinases that are responsible for the phosphorylation of several cellular targets. The B cyclins, when combined with p34cdc2, a catalytic subunit of the cell cycle dependent kinase, form an enzyme that is active at the transition to the G2 phase, attaining maximum level at M phase. At that phase, the complex associates with condensed chromosomes in prophase and metaphase and is degraded at the metaphase/anaphase transition.
      Anti-Cyclin B1 may be used for the detection of cyclin B1 expression and for measuring relative differences in cyclin B1 level as a function of cell cycle phase.
      References
      Product Information
      FormatPhycoerythrin
      Control
      • Jurkat Cells
      PresentationProtein G purified mouse IgGs conjugated to phycoerythrin in storage buffer PBS, 15mg/mL BSA and 0.1% sodium azide with out Glycerol.
      Applications
      ApplicationThis Milli-Mark Anti-Cyclin B1-PE Antibody, clone GNS3 (8A5D12) is validated for use in WB, IP, FC for the detection of Cyclin B1.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Flow Cytometry
      Biological Information
      ImmunogenRecombinant human cyclin B1
      EpitopeUnknown
      CloneGNS3 (8A5D12)
      HostMouse
      SpecificityThis antibody is specific for human cyclin B1, MW 58 kDa. Does not cross-react with cyclin A or cyclin D.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteTested and passed on Human. In addition to human, weak species cross-reactivity was observed with mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
      Gene Symbol
      • G2/mitotic-specific cyclin B1
      • cyclin B1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14635 # Essential for the control of the cell cycle at the G2/M (mitosis) transition.
      SIZE: 433 amino acids; 48337 Da
      SUBUNIT: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Centrosome.
      DEVELOPMENTAL STAGE: Accumulates steadily during G2 and is abruptly destroyed at mitosis.
      PTM: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
      SIMILARITY: SwissProt: P14635 ## Belongs to the cyclin family. Cyclin AB subfamily.
      Molecular Weight58 kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry on Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cyclin B1, clone GNS3 (8A5D12) PE Conjugate - NRG1677937 NRG1677937
      Milli-Mark Anti-Cyclin B1, clone GNS3 (8A5D12) PE - 2154105 2154105
      Milli-Mark Anti-Cyclin B1, clone GNS3 (8A5D12) PE - 2054814 2054814
      Milli-Mark Anti-Cyclin B1, clone GNS3 (8A5D12) PE - 2073071 2073071
      Milli-Mark Anti-Cyclin B1, clone GNS3 (8A5D12) PE - 2095630 2095630
      Milli-Mark Anti-Cyclin B1, clone GNS3 (8A5D12) PE - 2196062 2196062
      Milli-Mark Anti-Cyclin B1, clone GNS3 (8A5D12) PE - NG1818625 NG1818625
      Milli-Mark Anti-Cyclin B1, clone GNS3 (8A5D12) PE - NG1912441 NG1912441
      Milli-Mark™ Anti-Cyclin B1 2474947
      Milli-Mark™ Anti-Cyclin B1, clone GNS3 (8A5D12) PE -2712465 2712465

      Brochure

      Title
      Benchtop Flow Cytometry Solutions Kits and Reagents
      Innovative Solutions for Flow Cytometry Analysis

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      Alternative Format

      Catalogue Number Description  
      05-373 Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12) Show Pricing & Availability

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies