FCMAB129CP Milli-Mark Anti-EGFR-PerCP Antibody, clone LA22

FCMAB129CP
100 tests  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMPerCPMonoclonal Antibody
      Description
      Catalogue NumberFCMAB129CP
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-EGFR-PerCP Antibody, clone LA22
      Alternate Names
      • Receptor tyrosine-protein kinase ErbB-1
        avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
        cell growth inhibiting protein 40
        cell proliferation-inducing protein 61
        epidermal growth factor receptor
        epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog)
        epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
      Background InformationProtein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
      References
      Product Information
      FormatPerCP
      Control
      • A431 cells.
      PresentationPurified mouse monoclonal IgG2a conjugated to PerCP in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Applications
      ApplicationMilli-Mark Anti-EGFR-PerCP Antibody, clone LA22 detects level of EGFR & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Working Solution Cells / test Total Reaction Volume
      5 μl 5 x 105 Cells / 95 μl PBS 100 μl
      10 μl 1 x 106 Cells / 90 μl PBS 100 μl
      Biological Information
      ImmunogenA431 membrane proteins
      EpitopeUnknown
      CloneLA22
      HostMouse
      SpecificityRecognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kDa, (based on numbering without the signal peptide) (Wu et al., 1989).
      IsotypeIgG2a
      Species Reactivity
      • Human
      Species Reactivity NoteTested and passed on Human. based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq].
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.

      SIZE: 1210 amino acids; 134277 Da

      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.

      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.

      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].

      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.

      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation
      Molecular Weight180 kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 oC protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Milli-Mark Anti-EGFR-PerCP, clone LA22 - 21190112119011
      Milli-Mark Anti-EGFR-PerCP, clone LA22 - 21236832123683
      Milli-Mark Anti-EGFR-PerCP, CloneLA22 - 22626982262698
      Milli-Mark Anti-EGFR-PerCP, clone LA22 - 19564281956428
      Milli-Mark Anti-EGFR-PerCP, clone LA22 - 20927202092720
      Milli-Mark Anti-EGFR-PerCP, clone LA22 - 21996492199649
      Milli-Mark Anti-EGFR-PerCP, clone LA22 - DAM1893165DAM1893165
      Milli-Mark Anti-EGFR-PerCP, clone LA22 - NG1925105NG1925105
      Milli-Mark Anti-EGFR-PerCP, clone LA22 - NRG1714615NRG1714615
      Milli-Mark Anti-EGFR-PerCP, clone LA22 - NRG1727167NRG1727167

      Brochure

      Title
      Innovative Solutions for Flow Cytometry Analysis

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