Key Spec Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|Presentation||Purified mouse monoclonal IgG2a conjugated to PerCP in PBS with 0.1% sodium azide and 15 mg/mL BSA.|
|Immunogen||A431 membrane proteins|
|Specificity||Recognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kDa, (based on numbering without the signal peptide) (Wu et al., 1989).|
|Species Reactivity Note||Tested and passed on Human. based on 100% sequence homology.|
|Antibody Type||Monoclonal Antibody|
|Entrez Gene Number|
|Entrez Gene Summary||The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq].|
|Purification Method||Protein G Purified|
|UniProt Summary||FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation
|Molecular Weight||180 kDa Observed|
|Safety Information according to GHS|
|Product Usage Statements|
|Quality Assurance||Evaluated by flow cytometry using A431 cells.|
|Storage and Shipping Information|
|Storage Conditions||Maintain refrigerated at 2-8 oC protected from light in undiluted aliquots for up to 6 months from date of receipt.|
|Material Size||100 tests|
|Milli-Mark Anti-EGFR-PerCP, clone LA22 - 2119011||2119011|
|Milli-Mark Anti-EGFR-PerCP, clone LA22 - 2123683||2123683|
|Milli-Mark Anti-EGFR-PerCP, CloneLA22 - 2262698||2262698|
|Milli-Mark Anti-EGFR-PerCP, clone LA22 - 1956428||1956428|
|Milli-Mark Anti-EGFR-PerCP, clone LA22 - 2092720||2092720|
|Milli-Mark Anti-EGFR-PerCP, clone LA22 - 2199649||2199649|
|Milli-Mark Anti-EGFR-PerCP, clone LA22 - DAM1893165||DAM1893165|
|Milli-Mark Anti-EGFR-PerCP, clone LA22 - NG1925105||NG1925105|
|Milli-Mark Anti-EGFR-PerCP, clone LA22 - NRG1714615||NRG1714615|
|Milli-Mark Anti-EGFR-PerCP, clone LA22 - NRG1727167||NRG1727167|
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