FCMAB330F | Milli-Mark™ Anti-Rac1-FITC Antibody, clone 23A8

FCMAB330F
100 tests  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, HFCMFITCMonoclonal Antibody
      Description
      Catalogue NumberFCMAB330F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-Rac1-FITC Antibody, clone 23A8
      Background InformationRac1 is a member of a RhoGTPase subfamily (Rac1-Rac4) that transduces extracellular signals from G-coupled protein receptors (GPCR), integrins and growth factor receptors to effector molecules that modulate multiple signaling pathways. While Rac1 supports the activation of MAPkinase pathways and gene activation, it’s most important functions include the regulation of cytoskeletal structures. Together with Rho (regulator of stress fibers) and Cdc42 (regulator of filopodia), Rac modulates the formation of focal adhesion (FA) complexes; membrane ruffles and lamellipodia that contribute to important cell functions related to cell attachment and movement.
      References
      Product Information
      FormatFITC
      Control
      • B35 cells
      PresentationPurified mouse monoclonal IgG2b conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Applications
      ApplicationMilli-Mark Anti-Rac1-FITC Antibody, clone 23A8 detects level of Rac1 & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenRecombinant protein containing the full length human Rac1.
      Clone23A8
      HostMouse
      SpecificityAntibody recognizes Rac1. Slight Rac2 cross-reactivity.
      IsotypeIgG2b
      Species Reactivity
      • Rat
      • Mouse
      • Human
      Species Reactivity NoteTested and passed on Rat. Predicted to react with Mouse based on 100% sequence homology. Predicted to react with Human based on immunogen design.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RAC1
      • MGC111543
      • ORFNames=MIG5
      • PAK1
      • MIG5
      • TC-25
      • p21-Rac1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P63000 # Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP- dependent manner, suggesting that the insertion does not completely abolish effector interaction.

      SIZE: 192 amino acids; 21450 Da

      SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2.

      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C- terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

      TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

      DOMAIN: SwissProt: P63000 The effector region mediates interaction with DEF6.

      SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
      Molecular Weight 21 kDa Calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using B35 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Milli-Mark#174; Anti-Rac1-FITC, clone 23A8 - NRG1919867 NRG1919867
      Milli-MarkTM Anti-Rac1-FITC, clone 23A8 - 2424148 2424148
      Milli-MarkTM Anti-Rac1-FITC, clone 23A8 - 2426540 2426540
      Milli-MarkTM Anti-Rac1-FITC, clone 23A8 - 2443462 2443462
      Milli-MarkTM Anti-Rac1-FITC, clone 23A8 - 2445858 2445858
      Milli-MarkTM Anti-Rac1-FITC, -2547224 2547224
      Milli-MarkTM Anti-Rac1-FITC, -2584067 2584067
      Milli-MarkTM Anti-Rac1-FITC, -2604833 2604833
      Milli-MarkTM Anti-Rac1-FITC, -2676706 2676706
      Milli-MarkTM Anti-Rac1-FITC, clone 23A8 - 2332575 2332575

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies