FCMAB228F | Milli-Mark™ Anti-mouse CD45R (B220)-FITC Antibody, clone RA3-3A1/6.1

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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFCRFITCMonoclonal Antibody
      Catalogue NumberFCMAB228F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-mouse CD45R (B220)-FITC Antibody, clone RA3-3A1/6.1
      Alternate Names
      • Receptor-type tyrosine-protein phosphatase C
      • Leukocyte common antigen
      • L-CA
      • Lymphocyte antigen 5
      • Ly-5
      • T200
      Background InformationThe interleukin-2 receptors consist of three polypeptides (α, β, γ), which in different combinations bind IL-2 (Ishii, 1995). The 55 kDa α-chain is called CD25. CD25 is strongly expressed on the surface of activated T cells (Uchiyama, 1981), and is also expressed on B cells stimulated with anti-IgM antibody, and on monocytes/macrophages stimulated with lipopolysaccharide (Sugamura, 1997). In normal peripheral blood a variable expression on lymphoid cells (about 3-25%) is observed. The specificity of Anti-CD25, ACT-1, is identical to that of CD25-clustered antibodies (Schwarting, 1987; Aziz, 1996).
      Product Information
      • Mouse splenocytes
      PresentationPurified rat monoclonal IgM conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      ApplicationMilli-Mark Anti-mouse CD45R (B220)-FITC Antibody, clone RA3-3A1/6.1 is an antibody against mouse CD45R (B220) for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenAnimals were immunized with the mouse cell line RAW 112. Spleen cells were fused with the S194/5.XX0.BU.1 myeloma.
      SpecificityAntibody recognizes CD45 isoform 1 also known as B220.
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ptprc
      • Ly-5
      Purification MethodIon Exchange
      UniProt Number
      UniProt SummaryFUNCTION: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN By similarity.

      CATALYTIC ACTIVITY: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

      SUBUNIT STRUCTURE: Interacts with SKAP1. Interacts with DPP4; the interaction is enhanced in an interleukin-12-dependent manner in activated lymphocytes. Binds GANAB and PRKCSH.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.

      DEVELOPMENTAL STAGE: Expression is restricted to the hematopoietic compartment of development.

      DOMAIN: The first PTPase domain interacts with SKAP1

      POST-TRANSLATIONAL MODIFICATION: Heavily N- and O-glycosylated.

      SEQUENCE SIMILARITIES: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. Contains 2 fibronectin type-III domains. Contains 2 tyrosine-protein phosphatase domains.
      Molecular Weight 144 kDa Calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using mouse splenocytes
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information