FCMAB253F | Milli-Mark™Anti-Src-FITC Antibody, clone GD11

FCMAB253F
100 tests  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMFITCMonoclonal Antibody
      Description
      Catalogue NumberFCMAB253F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™Anti-Src-FITC Antibody, clone GD11
      Background InformationSrc is a family of proto-oncogenic tyrosine kinases originally discovered by J. Michael Bishop and Harold E. Varmus. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry. Some tumor-inducing retroviruses (such as Rous sarcoma virus (RSV)), contain a gene called v-src (viral-sarcoma). It was found that the v-src gene in RSV is required for the formation of cancer. Src tyrosine kinases transmit integrin-dependent signals central to cell movement and proliferation. Hallmarks of v-src induced transformation are rounding of the cell and the formation of actin rich podosomes on the basal surface of the cell. These structures are correlated with increased invasiveness, a process thought to be essential for metastasis. v-src lacks the C-terminal inhibitory phosphorylation site, and is therefore constitutively active as opposed to normal src (c-src) which is only activated under certain circumstances where it is required (e.g. growth factor signaling). v-src is therefore an instructive example of an oncogene whereas c-src is a proto-oncogene.
      References
      Product Information
      FormatFITC
      Control
      • A431 cells.
      PresentationPurified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Applications
      ApplicationMilli-MarkAnti-Src-FITC Antibody, clone GD11 detects level of Src & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenRecombinant chicken pp60src purified from extracts of E. coli containing the plasmid plac-Src (Kuo, 2006)
      CloneGD11
      HostMouse
      SpecificityAntibody recognizes Human Src.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteTested and passed on Human. Unconjugated antibody 05-184 has shown to react to Avian, Mouse, Mink, and Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • SRC
      • c-src
      • p60-Src
      • SRC1
      • c-SRC
      • ASV
      • pp60c-src
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummarySIZE: 536 amino acids; 59835 Da

      SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.

      PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.

      SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight60 kDa Calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies